Thermo Scientific Pierce Protease Inhibitor XL Capsules, EDTA-free, contain a broad spectrum formulation of four protease inhibitors and each capsule is sufficient for 500 mL of solution.
Features of EDTA-free Protease Inhibitor XL Capsules:
• Broad spectrum formulation—contains AEBSF, bestatin, E-64, and pepstatin A for better protection versus PMSF
• Large volume format—one capsule is sufficient for 500 mL of solution
• Convenient—ready-to-use, dissolves clearly into solution within three minutes
• Compatible—use directly with Pierce protein assays
• EDTA-free—formulated without EDTA, a metalloproteinase inhibitor
Pierce Protease Inhibitor XL Capsules protect proteins from proteolytic degradation through inhibition of the serine proteases, cysteine proteases, aspartic acid proteases, and aminopeptidases that are typically present in cell lysate samples. The tablets contain AEBSF, bestatin, E-64, and pepstatin A. Each capsule is sufficient for 500 mL of solution, and is suitable for reagent preparation prior to protein extraction from tissue and cultured cells.
Proteases make up a large category of enzymes, including endopeptidases and exopeptidases, which catalyze the hydrolytic breakdown of proteins into peptides or amino acids. Proteases reside in cellular compartments, but upon cell lysis the proteases are released, resulting in the degradation of valuable proteins. For this reason, it is common practice to add multiple compounds known to inactivate or inhibit proteases to cell lysis buffers or cell extracts. PMSF is commonly used for protease protection in large volumes, but does not offer the same level of protection as the capsule formulation.
To use Pierce Protease Inhibitor XL Capsules, simply twist the capsule to open and empty the contents of one capsule into 500 mL of solution. The protease inhibitor formulation is compatible with most detergent-based cell lysis reagents, including Pierce cell lysis solutions. Pierce Protease Inhibitor XL Capsules do not contain phosphatase inhibitors. If necessary, phosphatase inhibitors, such as Pierce phosphatase inhibitors, can be added along with the protease inhibitor capsules.
Related products
Halt Protease and Phosphatase Inhibitor Cocktail, EDTA-free (100X)
Halt Phosphatase Inhibitor Cocktail, EDTA-free (100X)
T-PER Tissue Protein Extraction Reagent
For Research Use Only. Not for use in diagnostic procedures.
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