久久亚洲精品天码av,午夜福利屄插穴,午夜福利视频91,亚欧美爽爽爽爽爽,亚洲欧美日韩色色图片,青青操在线观看,精品天堂麻豆av,无码av东京热,婷婷成人亚洲少妇

行業(yè)產(chǎn)品

  • 行業(yè)產(chǎn)品

賽默飛世爾科技（中國）有限公司


當(dāng)前位置：賽默飛世爾科技（中國）有限公司>>蛋白分析試劑>>蛋白純化>>Bac-to-Bac™ HBM TOPO™ 分泌表達(dá)系統(tǒng)

Bac-to-Bac™ HBM TOPO™ 分泌表達(dá)系統(tǒng)

返回列表頁
參  考  價面議
具體成交價以合同協(xié)議為準(zhǔn)

產(chǎn)品型號Gibco

品       牌

廠商性質(zhì)生產(chǎn)商

所  在  地

聯(lián)系方式：Tracy查看聯(lián)系方式

更新時間：2024-08-13 08:20:24瀏覽次數(shù)：190次

聯(lián)系我時，請告知來自 制藥網(wǎng)


    暫無信息

經(jīng)營模式：生產(chǎn)廠家

商鋪產(chǎn)品：1323條

所在地區(qū)：上海上海市

聯(lián)系人：Tracy （市場）

產(chǎn)品簡介

Bac-to-Bac™ HBM TOPO™ 分泌表達(dá)系統(tǒng)

詳細(xì)介紹

Bac-to-Bac baculovirus expression is an efficient method for producing baculovirus for high-level protein expression in insect cells. It relies on generation of recombinant virus by site-specific transposition in E. coli rather than homologous recombination in insect cells. The pFastBac HBM TOPO expression system features:

? Expression of secreted proteins—The Bac-to-Bac HBM TOPO vector contains the honeybee melittin (HBM) secretion signal to enable secreted protein expression. Because glycoproteins cannot be glycosylated in the absence of a secretion signal, this vector is recommended for the expression of glycoproteins.

? Flexibility—The Bac-to-Bac HBM TOPO vector contains a C-terminal His-tag with a TEV cleavage site to enable easy purification of His-tagged proteins with nickel-chelating resins (such as the Invitrogen ProBond Purification System) and generation of native proteins with the aid of the Invitrogen AcTEV Protease.

? Fast cloning, easy screening—Blunt TOPO cloning technology enables cloning of blunt-end PCR products into the pFastBac TOPO vector in five minutes. In addition, the pFastBac TOPO expression kit is supplied with Mach1-T1R E. coli that enable the visualization of colonies eight hours after plating on ampicillin-selective plates due to their faster doubling time compared to other standard cloning strains. Find out more about the advantages of TOPO cloning ›

? High transfection efficiency with ExpiFectamine Sf Transfection Reagent—The Bac-to-Bac TOPO expression kits now come with the next-generation ExpiFectamine Sf Transfection Reagent for efficient DNA transfection in insect cells using fast, flexible protocols. Find out more about this reagent ›

? Time-saving expression bacmid—With the Bac-to-Bac system, the expression cassette of the pFastBac vector recombines with the parent bacmid in DH10Bac E. coli Competent Cells to form an expression bacmid. The bacmid is then transfected into insect cells for production of recombinant baculovirus particles.

? Easy colony screening—The parent bacmid in DH10Bac E. coli contains a segment of the lacZa gene. The lacZa gene is disrupted upon transposition of the expression cassette into the bacmid, allowing for blue/white selection of recombinants for easier identification of recombinant colonies.

? High protein expression—The pFastBac vector uses the strong polyhedrin promoter to generate high levels of expression in a variety of insect cell line such as Sf9, Sf21, and High Five cells.

For Research Use Only. Not for use in diagnostic procedures.


您感興趣的產(chǎn)品PRODUCTS YOU ARE INTERESTED IN

制藥網(wǎng) 設(shè)計制作，未經(jīng)允許翻錄必究 .? ? ? Copyright(C)?2021 http://www.jjhengye.com,All rights reserved.

以上信息由企業(yè)自行提供，信息內(nèi)容的真實性、準(zhǔn)確性和合法性由相關(guān)企業(yè)負(fù)責(zé)，制藥網(wǎng)對此不承擔(dān)任何保證責(zé)任。 溫馨提示：為規(guī)避購買風(fēng)險，建議您在購買產(chǎn)品前務(wù)必確認(rèn)供應(yīng)商資質(zhì)及產(chǎn)品質(zhì)量。

會員登錄

×

請輸入賬號

請輸入密碼

=

請輸驗證碼

收藏該商鋪

請 登錄 后再收藏

提示

您的留言已提交成功！我們將在第一時間回復(fù)您~
蕲春县| 黄冈市| 邵阳市| 义乌市| 阿克陶县| 雅江县| 城市| 来安县| 嘉禾县| 二手房| 克山县| 崇信县| 通江县| 通江县| 嘉义市| 乌兰县| 曲松县| 敖汉旗| 洞口县| 富民县| 尚义县| 邵阳市| 聊城市| 邢台市| 罗源县| 北流市| 霍州市| 方正县| 彝良县| 郯城县| 杭锦后旗| 集安市| 滁州市| 靖安县| 桃江县| 北海市| 洛隆县| 咸宁市| 绥芬河市| 乌海市| 武乡县|