The QuantiGene Plex Mouse Housekeeping Gene Panel, 10-plex, enables measurement of the expression of relevant mouse housekeeper genes through the analysis of 10 RNA targets and biomarkers in a single well using Luminex xMAP technology in combination with branched DNA signal amplification. This panel contains housekeeping genes that are commonly used as reference genes in gene expression studies for reliable normalization and quality control.
QuantiGene Plex panels are extensively tested for target combinability, interference, and cross-reactivity to provide the highest levels of validation and precision.
This panel is used in combination with the QuantiGene Plex Assay Kit, which contains all buffers, plates, and detection reagents necessary to perform the assay in 96-well plates. For detailed information, see QuantiGene Plex Gene Expression Assay Ordering.
Features of QuantiGene Plex panels include:
• True multiplexing—measure multiple genes of interest and housekeeping genes in the same well with no cross-reactivity
• Accommodation of difficult sample types—works with degraded and cross-linked RNA in FFPE tissues and blood
• Standardized platform—uses 96-plate format compatible with Luminex 200, FLEXMAP 3D, and INTELLIFLEX systems (384-well format available on demand)
• Simple workflow—employs an ELISA-like workflow with direct hybridization of transcripts to beads and transcript labeling (branched DNA signal amplification and PE readout)
• Customize with validated targets—if alternative targets are needed in this panel, select from our large inventory of over 22,000 genes to create custom research panels
• Rapid turnaround—of this catalog panel for fast delivery
Targets: Actb, Gapdh, Gusb, Hprt, Tbp, Ppib, Polr2a, Rpl13a, Rpl19, Hmbs
Panel and signal amplification technology
QuantiGene Plex assays are hybridization-based assays for the direct quantitation of RNA directly from cell lysates, tissue homogenates, or purified RNA, and they enable the measurement of multiple targets in the same well via multiplexing. QuantiGene Plex panels contain two components: a target-specific probe set and magnetic capture beads. The target-specific probes are used to capture the RNA of interest, and branched DNA technology then amplifies the fluorescent signals, which are read with a Luminex xMAP instrument. The intensity of the fluorescent signal is directly proportional to the amount of target mRNA present in the sample. This allows for (relative) quantification of gene expression and, with this specific panel, enables human immune profiling. Detailed and step-by-step data analysis is facilitated by the QuantiGene Plex data analysis app.
Applications
QuantiGene Plex assay research applications include compound screening of drug candidates, biomarker verification, siRNA knockdown efficiency determination, prospective and/or retrospective analysis of clinical samples, microarray validation, predictive toxicology research, and translocation/fusion gene detection. In general, any multiplex gene expression analysis can be done with QuantiGene Plex assays, independent of species or sample type.
For Research Use Only. Not for use in diagnostic procedures.
會員1.png)
福贡县|
五家渠市|
陆川县|
志丹县|
教育|
浦城县|
长沙市|
皮山县|
嘉荫县|
庆城县|
乌鲁木齐县|
尤溪县|
五家渠市|
虎林市|
夏津县|
凤阳县|
西峡县|
南宁市|
肥东县|
二连浩特市|
县级市|
安岳县|
开封市|
郎溪县|
四平市|
富民县|
定西市|
河南省|
和静县|
神农架林区|
兴化市|
托克逊县|
涪陵区|
安溪县|
北海市|
绥德县|
江北区|
大姚县|
广饶县|
平乐县|
和平县|