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產(chǎn)品簡介

FAM hydrazide,酰肼綠色熒光染料
目前Cyanine系列染料以兩種異構(gòu)體的形式存在，一種是非磺化花青素（non-sulfonated cyanines），一種是磺化花青素（sulfonatedcyanine）。兩者的光譜性質(zhì)幾乎*，因此大部分應(yīng)用可相互替換。

詳細介紹

FAM hydrazide,酰肼綠色熒光染料

產(chǎn)品介紹

FAM hydrazide, 5-isomer

Many natural and synthetic molecules contain aldehyde or ketone carbonyl groups. These carbonyls react with hydrazides with the formation of hydrazones. The reaction is spontaneous at pH values around neutral, and the resulting hydrazones are very stable.

pounds containing 1,2-diol function, like sugars, can be oxidized with sodium periodate with the formation of carbonyl pounds for the subseuent modification with hydrazides. This is an efficient method for the labeling of glycoproteins (like antibodies), and polysaccharides.

FAM hydrazide is a hydrazide label for the attachment of fluorescein, a bright dye for 488 nm channel, to carbonyl pounds.

General properties

Appearance:	yellow solid
Mass spec M+ increment:	372.07
Molecular weight:	426.81
Molecular formula:	C₂₁H₁₅N₂ClO₆
Solubility:	good in ethanol, DMF, DMSO
*uality contol:**	NMR ¹H, HPLC-MS (95%)
Storage conditions:	Storage: 24 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light. Desiccate.

Spectral properties

Excitation maximum, nm:	494
ε, L⋅mol⁻¹⋅cm⁻¹:	75000
Emission maximum, nm:	520
Fluorescence uantum yield:	0.9
CF₂₆₀:	0.20
CF₂₈₀:	0.17