FAM tetrazine,綠光熒光染料標(biāo)記四嗪
目前Cyanine系列染料以兩種異構(gòu)體的形式存在，一種是非磺化花青素（non-sulfonated cyanines），一種是磺化花青素（sulfonatedcyanine）。兩者的光譜性質(zhì)幾乎*，因此大部分應(yīng)用可相互替換。

FAM tetrazine,綠光熒光染料標(biāo)記四嗪

產(chǎn)品介紹

FAM tetrazine, 5-isomer

Tetrazine (methyltetrazine) derivative of fluorescein, pure 5-isomer.

Tetrazines are reactive heterodiene ponents for [4+2] inverse electron demand Diels-Alder reaction (ied-DA) with strained cycloalkenes (trans-cyclooctenes, cyclopropenes). This reaction is also called TCO (tetrazine-cyclooctene) ligation. The reaction is among the fastest bioconjugation reactions.

General properties

Appearance:	yellow solid
Mass spec M+ increment:	529.12
Molecular weight:	559.53
Molecular formula:	C31H21N5O6
Solubility:	good in DMF, DMSO
uality cont*ol:	NMR 1H, HPLC-MS (95%)
Storage conditions:	Storage: 24 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light. Desiccate.

Spectral properties

Excitation maximum, nm:	490
ε, L⋅mol−1⋅cm−1:	80000
Emission maximum, nm:	513
Fluorescence uantum yield:	0.93
CF260:	0.20
CF280:	0.17

結(jié)構(gòu)式：

 

FAM tetrazine,綠光熒光染料標(biāo)記四嗪

N-羥基琥珀酰亞胺脂Dye NHS esters

Pyrenebutyric acid NHS ester

ROX NHS ester, pure 6- isomer

BDP FL NHS ester

FAM NHS ester

Cyanine3 NHS ester

Cyanine3.5 NHS ester

Cyanine5 NHS ester

Cyanine5.5 NHS ester

Cyanine7 NHS ester

Cyanine7.5 NHS ester

Sulfo-Cyanine3 NHS ester

Sulfo-Cyanine5 NHS ester

sulfo-Cyanine5 bis-NHS ester

Sulfo-Cyanine7 NHS ester