SF Cy5-SE|磺酸基-Cy5-N-羥基琥珀酰亞胺酯
水溶Cyanine 5琥珀酰亞胺酯（succinimidyl ester（SE）），類似于Cy5® NHS ester，不需要有機(jī)溶劑即在水相中即可對各種帶氨基分子進(jìn)行標(biāo)記。因此該產(chǎn)品尤其適用于對那些在有機(jī)溶劑中易變性的蛋白和低溶解度蛋白的標(biāo)記。

SF Cy5-SE|磺酸基-Cy5-N-羥基琥珀酰亞胺酯

 

水溶Cyanine 5琥珀酰亞胺酯（succinimidyl ester（SE）），類似于Cy5® NHS ester，不需要有機(jī)溶劑即在水相中即可對各種帶氨基分子進(jìn)行標(biāo)記。因此該產(chǎn)品尤其適用于對那些在有機(jī)溶劑中易變性的蛋白和低溶解度蛋白的標(biāo)記。

Sulfo-Cyanine 5是Cy5®的衍生物，與很多儀器如讀板機(jī)（plate readers），顯微鏡和成像設(shè)備等相兼容。是的熒光染料之一。

 該染料是高度親水的、水溶的。非磺化的衍生物：Cyanine 5 NHS ester。

可以在所有的應(yīng)用中替代Cy5®，Alexa Fluor 647和DyLight 649。

       SF Cy5-SE|磺酸基-Cy5-N-羥基琥珀酰亞胺酯     

 

 

一般特性

外觀:	dark blue powder
分子量:	761.84
分子式:	C36H40N3NaO10S2
溶解性:	very good in water, good in DMF and DMSO
質(zhì)量控制:	NMR 1H (95%) and 13C, TLC, functional testing
儲存條件:	Storage: 24 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light. Desiccate.

 

光譜特性

zui大激發(fā)波長, nm:	646
在zui大激發(fā)波長下的消光系數(shù), Lmol-1cm-1:	271000
zui大發(fā)生波長, nm:	662
熒光量子產(chǎn)率:	0.2
CF260:	0.13
CF280:	0.13

 

Cy系列的活性基團(tuán)菁染料：

N-羥基琥珀酰亞胺脂Dye NHS esters

Pyrenebutyric acid NHS ester

ROX NHS ester, pure 6- isomer

BDP FL NHS ester

FAM NHS ester

Cyanine3 NHS ester

Cyanine3.5 NHS ester

Cyanine5 NHS ester

Cyanine5.5 NHS ester

Cyanine7 NHS ester

Cyanine7.5 NHS ester

Sulfo-Cyanine3 NHS ester

Sulfo-Cyanine5 NHS ester

sulfo-Cyanine5 bis-NHS ester

Sulfo-Cyanine7 NHS ester

馬來酰亞胺 Dye maleimides

Pyrene maleimide

BDP FL maleimide

Cyanine3 maleimide

Cyanine5 maleimide

Cyanine5.5 maleimide

Cyanine7 maleimide

Cyanine7.5 maleimide

Sulfo-Cyanine3 maleimide

Sulfo-Cyanine5 maleimide

伯胺 Primary amine

BDP FL amine

Cyanine3 amine

Cyanine5 amine

Cyanine5.5 amine

Cyanine7 amine

Cyanine7.5 amine

Sulfo-Cyanine5 amine

Sulfo-Cyanine7 amine

酰肼 Dye hydrazides

Pyrene hydrazide

BDP FL hydrazide

Cyanine3 hydrazide

Cyanine5 Boc-hydrazide

Cyanine5 hydrazide

Cyanine5.5 hydrazide

Cyanine7 hydrazide

Cyanine7.5 hydrazide

馬來酰亞胺 Dye maleimides

BDP FL maleimide

Cyanine3 maleimide

Cyanine5 maleimide

Cyanine5.5 maleimide

Cyanine7 maleimide

Cyanine7.5 maleimide

Sulfo-Cyanine3 maleimide

Sulfo-Cyanine5 maleimide

羧酸 Carboxylic acids

Pyrenebutyric acid

BDP FL carboxylic acid

Cyanine3 carboxylic acid

Cyanine3.5 carboxylic acid

Cyanine5 carboxylic acid

Cyanine5.5 carboxylic acid

Cyanine7 carboxylic acid

Cyanine7.5 carboxylic acid

Sulfo-Cyanine3 carboxylic acid

Sulfo-Cyanine5 carboxylic acid

Sulfo-Cyanine7 carboxylic acid

點擊化學(xué)試劑

非熒光炔烴 Non-fluorescent alkynes

Pentynoic acid STP ester

Biotin alkyne

非熒光疊氮化物 Non-fluorescent azides

Azidobutyric acid NHS ester

Biotin azide

寡核苷酸分析

亞磷酰胺 Phosphoramidites

FAM Phosphoramidite, 6-Isomer

Alkyne Amidite, hydroxyprolinol

Alkyne Phosphoramidite, 5'-terminal

DMS(O)MT aminolink C6

Long trebler phosphoramidite

Pyrene phosphoramidite dU

PCR/PCR PCR/PCR

ROX reference dye for PCR

SYBR Green I Gel Staining Solution, 10000x

SYBR Green I for Real Time PCR, 100x

CPG固相修飾 CPG solid supports

Alkyne CPG modifier 500

TFA-Amino modifier CPG 500

三磷酸酯修飾 Modified triphosphates

Ami1-ddUTP

Ami1-dUTP