Sulfo-Cyanine5 NHS ester|146368-14-1
水溶Cyanine 5琥珀酰亞胺酯（succinimidyl ester（SE）），類似于Cy5® NHS ester，不需要有機溶劑即在水相中即可對各種帶氨基分子進行標記。因此該產(chǎn)品尤其適用于對那些在有機溶劑中易變性的蛋白和低溶解度蛋白的標記。

Sulfo-Cyanine5 NHS ester|146368-14-1

 

水溶Cyanine 5琥珀酰亞胺酯（succinimidyl ester（SE）），類似于Cy5® NHS ester，不需要有機溶劑即在水相中即可對各種帶氨基分子進行標記。因此該產(chǎn)品尤其適用于對那些在有機溶劑中易變性的蛋白和低溶解度蛋白的標記。

Sulfo-Cyanine 5是Cy5®的衍生物，與很多儀器如讀板機（plate readers），顯微鏡和成像設(shè)備等相兼容。是的熒光染料之一。

 該染料是高度親水的、水溶的。非磺化的衍生物：Cyanine 5 NHS ester。

可以在所有的應(yīng)用中替代Cy5®，Alexa Fluor 647和DyLight 649。

Sulfo-Cyanine5 NHS ester|146368-14-1       

 

 

一般特性

外觀:	dark blue powder
分子量:	761.84
分子式:	C36H40N3NaO10S2
溶解性:	very good in water, good in DMF and DMSO
質(zhì)量控制:	NMR 1H (95%) and 13C, TLC, functional testing
儲存條件:	Storage: 24 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light. Desiccate.

 

光譜特性

zui大激發(fā)波長, nm:	646
在zui大激發(fā)波長下的消光系數(shù), Lmol-1cm-1:	271000
zui大發(fā)生波長, nm:	662
熒光量子產(chǎn)率:	0.2
CF260:	0.13
CF280:	0.13

 

Cy系列的活性基團菁染料：

N-羥基琥珀酰亞胺脂Dye NHS esters

Pyrenebutyric acid NHS ester

ROX NHS ester, pure 6- isomer

BDP FL NHS ester

FAM NHS ester

Cyanine3 NHS ester

Cyanine3.5 NHS ester

Cyanine5 NHS ester

Cyanine5.5 NHS ester

Cyanine7 NHS ester

Cyanine7.5 NHS ester

Sulfo-Cyanine3 NHS ester

Sulfo-Cyanine5 NHS ester

sulfo-Cyanine5 bis-NHS ester

Sulfo-Cyanine7 NHS ester

馬來酰亞胺 Dye maleimides

Pyrene maleimide

BDP FL maleimide

Cyanine3 maleimide

Cyanine5 maleimide

Cyanine5.5 maleimide

Cyanine7 maleimide

Cyanine7.5 maleimide

Sulfo-Cyanine3 maleimide

Sulfo-Cyanine5 maleimide

伯胺 Primary amine

BDP FL amine

Cyanine3 amine

Cyanine5 amine

Cyanine5.5 amine

Cyanine7 amine

Cyanine7.5 amine

Sulfo-Cyanine5 amine

Sulfo-Cyanine7 amine

酰肼 Dye hydrazides

Pyrene hydrazide

BDP FL hydrazide

Cyanine3 hydrazide

Cyanine5 Boc-hydrazide

Cyanine5 hydrazide

Cyanine5.5 hydrazide

Cyanine7 hydrazide

Cyanine7.5 hydrazide

馬來酰亞胺 Dye maleimides

BDP FL maleimide

Cyanine3 maleimide

Cyanine5 maleimide

Cyanine5.5 maleimide

Cyanine7 maleimide

Cyanine7.5 maleimide

Sulfo-Cyanine3 maleimide

Sulfo-Cyanine5 maleimide

羧酸 Carboxylic acids

Pyrenebutyric acid

BDP FL carboxylic acid

Cyanine3 carboxylic acid

Cyanine3.5 carboxylic acid

Cyanine5 carboxylic acid

Cyanine5.5 carboxylic acid

Cyanine7 carboxylic acid

Cyanine7.5 carboxylic acid

Sulfo-Cyanine3 carboxylic acid

Sulfo-Cyanine5 carboxylic acid

Sulfo-Cyanine7 carboxylic acid

點擊化學(xué)試劑

非熒光炔烴 Non-fluorescent alkynes

Pentynoic acid STP ester

Biotin alkyne

非熒光疊氮化物 Non-fluorescent azides

Azidobutyric acid NHS ester

Biotin azide

寡核苷酸分析

亞磷酰胺 Phosphoramidites

FAM Phosphoramidite, 6-Isomer

Alkyne Amidite, hydroxyprolinol

Alkyne Phosphoramidite, 5'-terminal

DMS(O)MT aminolink C6

Long trebler phosphoramidite

Pyrene phosphoramidite dU

PCR/PCR PCR/PCR

ROX reference dye for PCR

SYBR Green I Gel Staining Solution, 10000x

SYBR Green I for Real Time PCR, 100x

CPG固相修飾 CPG solid supports

Alkyne CPG modifier 500

TFA-Amino modifier CPG 500

三磷酸酯修飾 Modified triphosphates

Ami1-ddUTP

Ami1-dUTP