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花菁類(lèi)染料（Cyanine dyes）的分類(lèi)介紹及選用指南

發(fā)布時(shí)間：2018-8-10

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花菁類(lèi)染料（Cyanine dyes）的分類(lèi)介紹及選用指南

花菁這類(lèi)染料的分子內(nèi)部含有由甲川基(CH)n組成的共軛鏈，n可為奇數(shù)或偶數(shù)。共軛鏈兩端或鏈中間連有雜環(huán)、芳環(huán)化合物、環(huán)烯化合物等與共軛鏈組成一個(gè)大的共軛體系，分子內(nèi)部的氫可被一定數(shù)目的各類(lèi)取代基取代。

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基于其結(jié)構(gòu)，花菁通常有非常高的超過(guò)100000 Lmol^-1cm^-1消光系數(shù)。不同的取代基具有控制發(fā)色團(tuán)的性能，如吸收波長(zhǎng)、耐光性、熒光。例如，通過(guò)聚次甲基橋鍵長(zhǎng)度的選擇來(lái)控制吸光度和熒光波長(zhǎng)：聚次甲基橋鍵越長(zhǎng)其吸光度越高且發(fā)射波長(zhǎng)可達(dá)到近紅外區(qū)。

已經(jīng)有許多的花菁染料（Cyanine dyes）用于生命科學(xué)領(lǐng)域，一系列的噻唑和惡唑染料用作DNA 和蛋白質(zhì)染色(如TOTO，YOYO， Stains All和其它)。但是在生命科學(xué)研究領(lǐng)域的花菁染料是由卡內(nèi)基梅隆大學(xué)的Alan Waggoner和他的同事們?cè)?990年代早期發(fā)現(xiàn)的。該花菁染料是經(jīng)過(guò)修飾后的吲哚菁綠(ICG)，從1970年代以來(lái)該染料一直用于血管造影術(shù)，而且他們都包含兩個(gè)假吲哚環(huán)側(cè)翼(CH)n鏈。發(fā)現(xiàn)此類(lèi)染料對(duì)生物分子表現(xiàn)出較低的非特異性結(jié)合，由于巨大的消光系數(shù)能發(fā)出明亮的熒光和良好的量子產(chǎn)量。一旦獲得，在卡內(nèi)基梅隆過(guò)期后這些分子可在公共領(lǐng)域獲得，并從Lumiprobe可購(gòu)買(mǎi)到，作為各種反應(yīng)衍生物用于科研和商業(yè)，比如用于點(diǎn)擊化學(xué)的NHS酯、馬來(lái)酰亞胺、疊氮化物的衍生品和其他衍生物。

花菁染料分為兩種：非磺化的花菁染料和磺化的花菁染料，對(duì)于許多的應(yīng)用，他們是可以互相替換的，因?yàn)樗麄兊墓庾V屬性幾乎相同?；腔头腔腔玖峡捎糜贒NA和蛋白質(zhì)等生物分子的標(biāo)記。兩種染料的區(qū)別在于它們的溶解度：磺基染料是水溶性，他們可在水環(huán)境中標(biāo)記無(wú)需添加有機(jī)助溶劑，他們?cè)谒锏姆稚⑿暂^好。在某些情況下，你可能會(huì)只需要這兩種染料中的一種（下面將具體講述非磺化的花菁染料和磺化的花菁染料的具體區(qū)別。）

非磺化的花菁染料

可用的非磺化的花菁染料包括Cy3，Cy3.5，Cy5，Cy5.5，Cy7，和Cy7.5，Cy代表‘cyanine’，*個(gè)數(shù)字代表了假吲哚之間的碳原子數(shù)。Cy2是惡唑衍生物而不是假吲哚，是不遵守這個(gè)規(guī)則的。后綴.5是苯丙稠合的花菁染料。結(jié)構(gòu)的變化也能改變分子的熒光特性，及覆蓋zui重要的可見(jiàn)光和攜帶幾個(gè)熒光基團(tuán)的近紅外光譜。

大部分非磺化的花菁染料衍生物（酰肼鹽酸鹽類(lèi)和胺類(lèi)除外）具有低水溶性，當(dāng)這些分子用于生物分子標(biāo)記時(shí)，使用有機(jī)溶劑作為助溶劑(5-20%的DMF或DMSO)是有效的反應(yīng)所必需的。花菁染料應(yīng)先溶解在有機(jī)溶劑中，之后在適當(dāng)?shù)乃芫彌_劑作用下加入到生物分子溶液中（蛋白質(zhì)，肽，氨基標(biāo)記的 DNA）。在染料沉淀之前，染料分子與生物分子發(fā)生有效反應(yīng)。

非磺化的花菁染料的熒光性質(zhì)對(duì)溶劑和反應(yīng)環(huán)境的依賴性較小，非磺化的的花菁染料的吸光度和熒光光譜繪制如下。

磺化的花菁染料

磺化的花菁染料包含了額外地磺基官能團(tuán)能促進(jìn)染料分子在水相中的溶解度，帶電荷的磺酸鹽降低了染料分子的聚合度且能很好地標(biāo)記偶聯(lián)物。

目前可用的磺化花菁染料包括sulfo-Cy3，sulfo-Cy5和sulfo-Cy7。

磺化花菁染料具有高度水溶性。標(biāo)記生物分子時(shí)不需要有機(jī)助溶劑輔助。

磺化和非磺化花菁染料之間的異同

磺化和非磺化花菁染料都展現(xiàn)出非常相似的熒光性能。然而，在使用說(shuō)明上有幾個(gè)不同點(diǎn)需要注意，非磺化花菁染料必須要先溶解在有機(jī)助溶劑中（DMF or DMSO），之后在適當(dāng)?shù)乃芫彌_劑作用下加入靶標(biāo)生物分子溶液。推薦使用的助溶劑的體積為，溶解Cy3， Cy5， Cy7使用10%助溶劑，.5的染料使用15%的助溶劑。而磺化的花菁染料可以在純粹的水環(huán)境中應(yīng)用。關(guān)于花菁染料在純化方面的差異：當(dāng)使用與水或水溶緩沖溶液相關(guān)的透析純化染料時(shí)，必須使用sulfo-Cy以實(shí)現(xiàn)除去未反應(yīng)的染料材料。與磺化和非磺化花菁染料的反應(yīng)可以通過(guò)凝膠過(guò)濾，色譜法 (FPLC，液相色譜法，離子交換法)，或電泳純化。

磺化和非磺化花菁染料在標(biāo)記以下類(lèi)別目標(biāo)時(shí)是可互換的:

1.可溶性蛋白，其對(duì)額外添加的有機(jī)助溶劑具有耐受性

2.抗體(使用5 - 10% DMSO / DMF)

3.DNA和寡核苷酸

4.肽

5.許多小分子

注：用類(lèi)似磺化和非磺化的試劑標(biāo)記的共軛物（例如：sulfo-Cy5 和Cy5）的熒光性質(zhì)是非常相似的，可用于各種熒光儀器。

以下情況必須選用磺化花菁染料：

1.遇DMF 或 DMSO變性的敏感蛋白質(zhì)

2.通過(guò)透析純化的蛋白質(zhì)偶聯(lián)物

3.在水溶液中有納米粒子

4.不溶于水或疏水的蛋白

以下情況必須選用非磺化花菁染料：

在有機(jī)相中發(fā)生的反應(yīng)（二氯甲烷，腈化甲烷）

西安凱新生物科技有限公司花菁染料類(lèi)染料列表：

脂溶性花菁染料：

Cyanine2 NHS ester minimal dye, 5 nmol

Cyanine2 NHS ester minimal dye, 10 nmol

Cyanine2 NHS ester minimal dye, 25 nmol

Cyanine3 alkyne, 1 mg

Cyanine3 alkyne, 5 mg

Cyanine3 alkyne, 10 mg

Cyanine3 alkyne, 25 mg

Cyanine3 alkyne, 50 mg

Cyanine3 alkyne, 100 mg

Cyanine3 amine, 1 mg

Cyanine3 amine, 5 mg

Cyanine3 amine, 25 mg

Cyanine3 amine, 50 mg

Cyanine3 amine, 100 mg

Cyanine3 azide, 100 uL, 10 mM/DMSO

Cyanine3 azide, 500 uL, 10 mM/DMSO

Cyanine3 azide, 1 mL, 10 mM/DMSO

Cyanine3 azide, 1 mg

Cyanine3 azide, 5 mg

Cyanine3 azide, 10 mg

Cyanine3 azide, 25 mg

Cyanine3 azide, 50 mg

Cyanine3 azide, 100 mg

Cyanine3 carboxylic acid, 1 mg

Cyanine3 carboxylic acid, 5 mg

Cyanine3 carboxylic acid, 25 mg

Cyanine3 carboxylic acid, 50 mg

Cyanine3 carboxylic acid, 100 mg

Cyanine3 DBCO, 1 mg

Cyanine3 DBCO, 5 mg

Cyanine3 DBCO, 10 mg

Cyanine3 DBCO, 25 mg

Cyanine3 DBCO, 50 mg

Cyanine3 DBCO, 100 mg

Cyanine3 hydrazide, 1 mg

Cyanine3 hydrazide, 5 mg

Cyanine3 hydrazide, 25 mg

Cyanine3 hydrazide, 50 mg

Cyanine3 hydrazide, 100 mg

Cyanine3 maleimide, 1 mg

Cyanine3 maleimide, 5 mg

Cyanine3 maleimide, 25 mg

Cyanine3 maleimide, 50 mg

Cyanine3 maleimide, 100 mg

Cyanine3 NHS ester, 5 mg

Cyanine3 NHS ester, 25 mg

Cyanine3 NHS ester, 50 mg

Cyanine3 NHS ester, 100 mg

Cyanine3 NHS ester minimal dye, 5 nmol

Cyanine3 NHS ester minimal dye, 10 nmol

Cyanine3 NHS ester minimal dye, 25 nmol

Cyanine3 tetrazine, 1 mg

Cyanine3 tetrazine, 5 mg

Cyanine3 tetrazine, 25 mg

Cyanine3 tetrazine, 50 mg

Cyanine3 tetrazine, 100 mg

Cyanine3.5 azide, 100 uL, 10 mM/DMSO

Cyanine3.5 azide, 500 uL, 10 mM/DMSO

Cyanine3.5 azide, 1 mL, 10 mM/DMSO

Cyanine3.5 azide, 1 mg

Cyanine3.5 azide, 5 mg

Cyanine3.5 azide, 10 mg

Cyanine3.5 azide, 25 mg

Cyanine3.5 azide, 50 mg

Cyanine3.5 carboxylic acid, 1 mg

Cyanine3.5 carboxylic acid, 5 mg

Cyanine3.5 carboxylic acid, 25 mg

Cyanine3.5 carboxylic acid, 50 mg

Cyanine3.5 carboxylic acid, 100 mg

Cyanine3.5 NHS ester, 1 mg

Cyanine3.5 NHS ester, 5 mg

Cyanine3.5 NHS ester, 25 mg

Cyanine3.5 NHS ester, 50 mg

Cyanine3.5 NHS ester, 100 mg

Cyanine5 alkyne, 1 mg

Cyanine5 alkyne, 5 mg

Cyanine5 alkyne, 10 mg

Cyanine5 alkyne, 25 mg

Cyanine5 alkyne, 50 mg

Cyanine5 alkyne, 100 mg

Cyanine5 amine, 1 mg

Cyanine5 amine, 5 mg

Cyanine5 amine, 25 mg

Cyanine5 amine, 50 mg

Cyanine5 amine, 100 mg

Cyanine5 azide, 100 uL, 10 mM/DMSO

Cyanine5 azide, 500 uL, 10 mM/DMSO

Cyanine5 azide, 1 mL, 10 mM/DMSO

Cyanine5 azide, 1 mg

Cyanine5 azide, 5 mg

Cyanine5 azide, 10 mg

Cyanine5 azide, 25 mg

Cyanine5 azide, 50 mg

Cyanine5 azide, 100 mg

Cyanine5 Boc-hydrazide, 1 mg

Cyanine5 Boc-hydrazide, 5 mg

Cyanine5 Boc-hydrazide, 25 mg

Cyanine5 Boc-hydrazide, 50 mg

Cyanine5 Boc-hydrazide, 100 mg

Cyanine5 carboxylic acid, 1 mg

Cyanine5 carboxylic acid, 5 mg

Cyanine5 carboxylic acid, 25 mg

Cyanine5 carboxylic acid, 50 mg

Cyanine5 carboxylic acid, 100 mg

Cyanine5 DBCO, 1 mg

Cyanine5 DBCO, 5 mg

Cyanine5 DBCO, 10 mg

Cyanine5 DBCO, 25 mg

Cyanine5 DBCO, 50 mg

Cyanine5 DBCO, 100 mg

Cyanine5 hydrazide, 1 mg

Cyanine5 hydrazide, 5 mg

Cyanine5 hydrazide, 25 mg

Cyanine5 hydrazide, 50 mg

Cyanine5 hydrazide, 100 mg

Cyanine5 maleimide, 1 mg

Cyanine5 maleimide, 5 mg

Cyanine5 maleimide, 25 mg

Cyanine5 maleimide, 50 mg

Cyanine5 maleimide, 100 mg

<="" a="">Cyanine5 NHS ester, 1 mg

Cyanine5 NHS ester, 5 mg

Cyanine5 NHS ester, 25 mg

Cyanine5 NHS ester, 50 mg

Cyanine5 NHS ester, 100 mg

Cyanine5 NHS ester minimal dye, 5 nmol

Cyanine5 NHS ester minimal dye, 10 nmol

Cyanine5 NHS ester minimal dye, 25 nmol

Cyanine5 tetrazine, 1 mg

Cyanine5 tetrazine, 5 mg

Cyanine5 tetrazine, 25 mg

Cyanine5 tetrazine, 50 mg

Cyanine5 tetrazine, 100 mg

Cyanine5.5 alkyne, 1 mg

Cyanine5.5 alkyne, 5 mg

Cyanine5.5 alkyne, 10 mg

Cyanine5.5 alkyne, 25 mg

Cyanine5.5 alkyne, 50 mg

Cyanine5.5 alkyne, 100 mg

Cyanine5.5 amine, 1 mg

Cyanine5.5 amine, 5 mg

Cyanine5.5 amine, 25 mg

Cyanine5.5 amine, 50 mg

Cyanine5.5 amine, 100 mg

Cyanine5.5 azide, 100 uL, 10 mM/DMSO

Cyanine5.5 azide, 500 uL, 10 mM/DMSO

Cyanine5.5 azide, 1 mL, 10 mM/DMSO

Cyanine5.5 azide, 1 mg

Cyanine5.5 azide, 5 mg

Cyanine5.5 azide, 10 mg

Cyanine5.5 azide, 25 mg

Cyanine5.5 azide, 50 mg

Cyanine5.5 carboxylic acid, 1 mg

Cyanine5.5 carboxylic acid, 5 mg

Cyanine5.5 carboxylic acid, 25 mg

Cyanine5.5 carboxylic acid, 50 mg

Cyanine5.5 carboxylic acid, 100 mg

Cyanine5.5 hydrazide, 1 mg

Cyanine5.5 hydrazide, 5 mg

Cyanine5.5 hydrazide, 25 mg

Cyanine5.5 hydrazide, 50 mg

Cyanine5.5 hydrazide, 100 mg

Cyanine5.5 maleimide, 1 mg

Cyanine5.5 maleimide, 5 mg

Cyanine5.5 maleimide, 25 mg

Cyanine5.5 maleimide, 50 mg

Cyanine5.5 maleimide, 100 mg

Cyanine5.5 NHS ester, 1 mg

Cyanine5.5 NHS ester, 5 mg

Cyanine5.5 NHS ester, 25 mg

Cyanine5.5 NHS ester, 50 mg

Cyanine5.5 NHS ester, 100 mg

Cyanine5.5 tetrazine, 1 mg

Cyanine5.5 tetrazine, 5 mg

Cyanine5.5 tetrazine, 25 mg

Cyanine5.5 tetrazine, 50 mg

Cyanine5.5 tetrazine, 100 mg

Cyanine7 alkyne, 1 mg

Cyanine7 alkyne, 5 mg

Cyanine7 alkyne, 10 mg

Cyanine7 alkyne, 25 mg

Cyanine7 alkyne, 50 mg

Cyanine7 alkyne, 100 mg

Cyanine7 amine, 1 mg

Cyanine7 amine, 5 mg

Cyanine7 amine, 25 mg

Cyanine7 amine, 50 mg

Cyanine7 amine, 100 mg

Cyanine7 azide, 100 uL, 10 mM/DMSO

Cyanine7 azide, 500 uL, 10 mM/DMSO

Cyanine7 azide, 1 mL, 10 mM/DMSO

Cyanine7 azide, 1 mg

Cyanine7 azide, 5 mg

Cyanine7 azide, 10 mg

Cyanine7 azide, 25 mg

Cyanine7 azide, 50 mg

Cyanine7 carboxylic acid, 1 mg

Cyanine7 carboxylic acid, 5 mg

Cyanine7 carboxylic acid, 25 mg

Cyanine7 carboxylic acid, 50 mg

Cyanine7 carboxylic acid, 100 mg

Cyanine7 DBCO, 1 mg

Cyanine7 DBCO, 5 mg

Cyanine7 DBCO, 10 mg

Cyanine7 DBCO, 25 mg

Cyanine7 DBCO, 50 mg

Cyanine7 DBCO, 100 mg

Cyanine7 dicarboxylic acid, 100 mg

Cyanine7 dicarboxylic acid, 1 mg

Cyanine7 dicarboxylic acid, 25 mg

Cyanine7 dicarboxylic acid, 50 mg

Cyanine7 dicarboxylic acid, 5 mg

Cyanine7 hydrazide, 1 mg

Cyanine7 hydrazide, 5 mg

Cyanine7 hydrazide, 25 mg

Cyanine7 hydrazide, 50 mg

Cyanine7 hydrazide, 100 mg

Cyanine7 maleimide, 1 mg

Cyanine7 maleimide, 5 mg

Cyanine7 maleimide, 25 mg

Cyanine7 maleimide, 50 mg

Cyanine7 maleimide, 100 mg

Cyanine7 NHS ester, 1 mg

Cyanine7 NHS ester, 5 mg

Cyanine7 NHS ester, 25 mg

Cyanine7 NHS ester, 50 mg

Cyanine7 NHS ester, 100 mg

Cyanine7 tetrazine, 1 mg

Cyanine7 tetrazine, 5 mg

Cyanine7 tetrazine, 25 mg

Cyanine7 tetrazine, 50 mg

Cyanine7 tetrazine, 100 mg

Cyanine7.5 alkyne, 1 mg

Cyanine7.5 alkyne, 5 mg

Cyanine7.5 alkyne, 10 mg

Cyanine7.5 alkyne, 25 mg

Cyanine7.5 alkyne, 50 mg

Cyanine7.5 alkyne, 100 mg

Cyanine7.5 amine, 1 mg

Cyanine7.5 amine, 5 mg

Cyanine7.5 amine, 10 mg

Cyanine7.5 amine, 25 mg

Cyanine7.5 amine, 50 mg

Cyanine7.5 amine, 100 mg

Cyanine7.5 azide, 100 uL, 10 mM/DMSO

Cyanine7.5 azide, 500 uL, 10 mM/DMSO

Cyanine7.5 azide, 1 mL, 10 mM in DMSO

Cyanine7.5 azide, 1 mg

Cyanine7.5 azide, 5 mg

Cyanine7.5 azide, 10 mg

Cyanine7.5 azide, 25 mg

Cyanine7.5 azide, 50 mg

Cyanine7.5 azide, 100 mg

Cyanine7.5 carboxylic acid, 1 mg

Cyanine7.5 carboxylic acid, 5 mg

Cyanine7.5 carboxylic acid, 25 mg

Cyanine7.5 carboxylic acid, 50 mg

Cyanine7.5 carboxylic acid, 100 mg

Cyanine7.5 hydrazide, 1 mg

Cyanine7.5 hydrazide, 5 mg

Cyanine7.5 hydrazide, 25 mg

Cyanine7.5 hydrazide, 50 mg

Cyanine7.5 hydrazide, 100 mg

Cyanine7.5 maleimide, 1 mg

Cyanine7.5 maleimide, 5 mg

Cyanine7.5 maleimide, 25 mg

Cyanine7.5 maleimide, 50 mg

Cyanine7.5 maleimide, 100 mg

Cyanine7.5 NHS ester, 5 mg

Cyanine7.5 NHS ester, 25 mg

Cyanine7.5 NHS ester, 50 mg

Cyanine7.5 NHS ester, 100 mg

Cyanine7.5 tetrazine, 1 mg

Cyanine7.5 tetrazine, 5 mg

Cyanine7.5 tetrazine, 25 mg

Cyanine7.5 tetrazine, 50 mg

Cyanine7.5 tetrazine, 100 mg

水溶性花菁染料：

Sulfo-Cyanine3 alkyne, 1 mg

Sulfo-Cyanine3 alkyne, 5 mg

Sulfo-Cyanine3 alkyne, 10 mg

Sulfo-Cyanine3 alkyne, 25 mg

Sulfo-Cyanine3 alkyne, 50 mg

Sulfo-Cyanine3 alkyne, 100 mg

Sulfo-Cyanine3 amine, 1 mg

Sulfo-Cyanine3 amine, 5 mg

Sulfo-Cyanine3 amine, 25 mg

Sulfo-Cyanine3 amine, 50 mg

Sulfo-Cyanine3 amine, 100 mg

Sulfo-Cyanine3 azide, 1 mg

Sulfo-Cyanine3 azide, 5 mg

Sulfo-Cyanine3 azide, 10 mg

Sulfo-Cyanine3 azide, 25 mg

Sulfo-Cyanine3 azide, 50 mg

Sulfo-Cyanine3 azide, 100 mg

Sulfo-Cyanine3 carboxylic acid, 1 mg

Sulfo-Cyanine3 carboxylic acid, 5 mg

Sulfo-Cyanine3 carboxylic acid, 25 mg

Sulfo-Cyanine3 carboxylic acid, 50 mg

Sulfo-Cyanine3 carboxylic acid, 100 mg

Sulfo-Cyanine3 maleimide, 1 mg

Sulfo-Cyanine3 maleimide, 5 mg

Sulfo-Cyanine3 maleimide, 25 mg

Sulfo-Cyanine3 maleimide, 50 mg

Sulfo-Cyanine3 maleimide, 100 mg

Sulfo-Cyanine3 NHS ester, 1 mg

Sulfo-Cyanine3 NHS ester, 5 mg

Sulfo-Cyanine3 NHS ester, 25 mg

Sulfo-Cyanine3 NHS ester, 50 mg

Sulfo-Cyanine3 NHS ester, 100 mg

Sulfo-Cyanine3 tetrazine, 1 mg

Sulfo-Cyanine3 tetrazine, 5 mg

Sulfo-Cyanine3 tetrazine, 10 mg

Sulfo-Cyanine3 tetrazine, 25 mg

Sulfo-Cyanine3 tetrazine, 50 mg

Sulfo-Cyanine3 tetrazine, 100 mg

Sulfo-Cyanine5 alkyne, 1 mg

Sulfo-Cyanine5 alkyne, 5 mg

Sulfo-Cyanine5 alkyne, 10 mg

Sulfo-Cyanine5 alkyne, 25 mg

Sulfo-Cyanine5 alkyne, 50 mg

Sulfo-Cyanine5 alkyne, 100 mg

Sulfo-Cyanine5 amine, 1 mg

Sulfo-Cyanine5 amine, 5 mg

Sulfo-Cyanine5 amine, 25 mg

Sulfo-Cyanine5 amine, 50 mg

Sulfo-Cyanine5 amine, 100 mg

Sulfo-Cyanine5 azide, 1 mg

Sulfo-Cyanine5 azide, 5 mg

Sulfo-Cyanine5 azide, 10 mg

Sulfo-Cyanine5 azide, 25 mg

Sulfo-Cyanine5 azide, 50 mg

Sulfo-Cyanine5 azide, 100 mg

Sulfo-Cyanine5 bis-NHS ester, 1 mg

Sulfo-Cyanine5 bis-NHS ester, 5 mg

Sulfo-Cyanine5 bis-NHS ester, 25 mg

Sulfo-Cyanine5 bis-NHS ester, 50 mg

Sulfo-Cyanine5 bis-NHS ester, 100 mg

Sulfo-Cyanine5 carboxylic acid, 1 mg

Sulfo-Cyanine5 carboxylic acid, 5 mg

Sulfo-Cyanine5 carboxylic acid, 25 mg

Sulfo-Cyanine5 carboxylic acid, 50 mg

Sulfo-Cyanine5 carboxylic acid, 100 mg

Sulfo-Cyanine5 dUTP, 250 nmol

Sulfo-Cyanine5 dUTP, 50 nmol

Sulfo-Cyanine5 maleimide, 1 mg

Sulfo-Cyanine5 maleimide, 5 mg

Sulfo-Cyanine5 maleimide, 25 mg

Sulfo-Cyanine5 maleimide, 50 mg

Sulfo-Cyanine5 maleimide, 100 mg

Sulfo-Cyanine5 NHS ester, 1 mg

Sulfo-Cyanine5 NHS ester, 5 mg

Sulfo-Cyanine5 NHS ester, 25 mg

Sulfo-Cyanine5 NHS ester, 50 mg

Sulfo-Cyanine5 NHS ester, 100 mg

Sulfo-Cyanine5 tetrazine, 1 mg

Sulfo-Cyanine5 tetrazine, 5 mg

Sulfo-Cyanine5 tetrazine, 10 mg

Sulfo-Cyanine5 tetrazine, 25 mg

Sulfo-Cyanine5 tetrazine, 50 mg

Sulfo-Cyanine5 tetrazine, 100 mg

Sulfo-Cyanine5.5 alkyne, 1 mg

Sulfo-Cyanine5.5 alkyne, 5 mg

Sulfo-Cyanine5.5 alkyne, 10 mg

Sulfo-Cyanine5.5 alkyne, 25 mg

Sulfo-Cyanine5.5 alkyne, 50 mg

Sulfo-Cyanine5.5 alkyne, 100 mg

Sulfo-Cyanine5.5 amine, 1 mg

Sulfo-Cyanine5.5 amine, 5 mg

Sulfo-Cyanine5.5 amine, 25 mg

Sulfo-Cyanine5.5 amine, 50 mg

Sulfo-Cyanine5.5 amine, 100 mg

Sulfo-Cyanine5.5 azide, 1 mg

Sulfo-Cyanine5.5 azide, 5 mg

Sulfo-Cyanine5.5 azide, 10 mg

Sulfo-Cyanine5.5 azide, 25 mg

Sulfo-Cyanine5.5 azide, 50 mg

Sulfo-Cyanine5.5 azide, 100 mg

Sulfo-Cyanine5.5 carboxylic acid, 1 mg

Sulfo-Cyanine5.5 carboxylic acid, 5 mg

Sulfo-Cyanine5.5 carboxylic acid, 25 mg

Sulfo-Cyanine5.5 carboxylic acid, 50 mg

Sulfo-Cyanine5.5 carboxylic acid, 100 mg

Sulfo-Cyanine5.5 maleimide, 1 mg

Sulfo-Cyanine5.5 maleimide, 5 mg

Sulfo-Cyanine5.5 maleimide, 25 mg

Sulfo-Cyanine5.5 maleimide, 50 mg

Sulfo-Cyanine5.5 maleimide, 100 mg

Sulfo-Cyanine5.5 NHS ester, 1 mg

Sulfo-Cyanine5.5 NHS ester, 5 mg

Sulfo-Cyanine5.5 NHS ester, 25 mg

Sulfo-Cyanine5.5 NHS ester, 50 mg

Sulfo-Cyanine5.5 NHS ester, 100 mg

Sulfo-Cyanine7 alkyne, 1 mg

Sulfo-Cyanine7 alkyne, 5 mg

Sulfo-Cyanine7 alkyne, 10 mg

Sulfo-Cyanine7 alkyne, 25 mg

Sulfo-Cyanine7 alkyne, 50 mg

Sulfo-Cyanine7 alkyne, 100 mg

Sulfo-Cyanine7 amine, 1 mg

Sulfo-Cyanine7 amine, 5 mg

Sulfo-Cyanine7 amine, 25 mg

Sulfo-Cyanine7 amine, 50 mg

Sulfo-Cyanine7 amine, 100 mg

Sulfo-Cyanine7 azide, 1 mg

Sulfo-Cyanine7 azide, 5 mg

Sulfo-Cyanine7 azide, 10 mg

Sulfo-Cyanine7 azide, 25 mg

Sulfo-Cyanine7 azide, 50 mg

Sulfo-Cyanine7 azide, 100 mg

Sulfo-Cyanine7 carboxylic acid, 1 mg

Sulfo-Cyanine7 carboxylic acid, 5 mg

Sulfo-Cyanine7 carboxylic acid, 25 mg

Sulfo-Cyanine7 carboxylic acid, 50 mg

Sulfo-Cyanine7 carboxylic acid, 100 mg

sulfo-Cyanine7 dicarboxylic acid, 100 mg

sulfo-Cyanine7 dicarboxylic acid, 1 mg

sulfo-Cyanine7 dicarboxylic acid, 25 mg

sulfo-Cyanine7 dicarboxylic acid, 50 mg

sulfo-Cyanine7 dicarboxylic acid, 5 mg

Sulfo-Cyanine7 maleimide, 1 mg

Sulfo-Cyanine7 maleimide, 5 mg

Sulfo-Cyanine7 maleimide, 25 mg

Sulfo-Cyanine7 maleimide, 50 mg

Sulfo-Cyanine7 maleimide, 100 mg

Sulfo-Cyanine7 NHS ester, 1 mg

Sulfo-Cyanine7 NHS ester, 5 mg

Sulfo-Cyanine7 NHS ester, 25 mg

Sulfo-Cyanine7 NHS ester, 50 mg

Sulfo-Cyanine7 NHS ester, 100 mg

Sulfo-Cyanine7.5 alkyne, 1 mg

Sulfo-Cyanine7.5 alkyne, 5 mg

Sulfo-Cyanine7.5 alkyne, 10 mg

Sulfo-Cyanine7.5 alkyne, 25 mg

Sulfo-Cyanine7.5 alkyne, 50 mg

Sulfo-Cyanine7.5 alkyne, 100 mg

Sulfo-Cyanine7.5 azide, 1 mg

Sulfo-Cyanine7.5 azide, 5 mg

Sulfo-Cyanine7.5 azide, 10 mg

Sulfo-Cyanine7.5 azide, 25 mg

Sulfo-Cyanine7.5 azide, 50 mg

Sulfo-Cyanine7.5 azide, 100 mg

Sulfo-Cyanine7.5 carboxylic acid, 1 mg

Sulfo-Cyanine7.5 carboxylic acid, 5 mg

Sulfo-Cyanine7.5 carboxylic acid, 25 mg

Sulfo-Cyanine7.5 carboxylic acid, 50 mg

Sulfo-Cyanine7.5 carboxylic acid, 100 mg

Sulfo-Cyanine7.5 dicarboxylic acid, 100 mg

Sulfo-Cyanine7.5 dicarboxylic acid, 1 mg

Sulfo-Cyanine7.5 dicarboxylic acid, 25 mg

Sulfo-Cyanine7.5 dicarboxylic acid, 50 mg

Sulfo-Cyanine7.5 dicarboxylic acid, 5 mg

Sulfo-Cyanine7.5 NHS ester, 1 mg

Sulfo-Cyanine7.5 NHS ester, 5 mg

Sulfo-Cyanine7.5 NHS ester, 25 mg

Sulfo-Cyanine7.5 NHS ester, 50 mg

Sulfo-Cyanine7.5 NHS ester, 100 mg

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資料下載

花菁類(lèi)染料（Cyanine dyes）的分類(lèi)介紹及選用指南

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