Dibenzocyclooctyne-聚乙二醇-葉酸 (DBCO-PEG-FA)

Dibenzocyclooctyne-聚乙二醇-葉酸 (DBCO-PEG-FA)

?（備注：非常規(guī)分子量：400、600。精確聚合度的小分子定制。）

結(jié)構(gòu)式：

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基本信息：

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中文名稱：Dibenzocyclooctyne-聚乙二醇-葉酸 (DBCO-PEG-FA)

別名：DBCO-PEG-FA

英文名稱：DBCO-PEG-FA，DBCO-PEG- Folic acid

CAS：-

分子量：400、600、1K、2K、3.4K、4K、5K、10K

純度：90%以上

應(yīng)用簡(jiǎn)介：

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DBCO PEG Folic acid, also called DBCO PEG folate, is one of Nanocs' folic acid PEG derivatives that can go Click Chemistry reaction without a need of any metal catalysts. The strain-promoted 1,3-dipolar cycloaddition of cyclooctynes and azides, also termed as the Cu-free click reaction, is a bioorthogonal reaction that enables the conjugation of two molecules in aqueous solution. DBCO PEG folate derivatives possess fast kinetics and stability in aqueous buffer. DBCO reagents can be used to label azide-modified biomolecules spontaneous without the need for toxic Cu catalysts. Folic acid, is also known as vitamin M, vitamin B9 or pteroyl-L-glutamic acid. Folic acid is an essential bioactive molecule for numerous biological functions. It participates in the synthesis, repairing and methylation of DNA as well as to act as a cofactor in many biological reactions. DBCO functional folic acid can be easily attached to azide functionalized molecules, particles or solid surfaces.

Copper-free Click Reaction Procedures:

Prepare the azide-containing sample in reaction buffer. Add DBCO conjugate to azide containing sample. Recommendation: Add 1 mole equivalent of limiting reagent to 1.5-3.0 mole equivalents of highest abundance reagent. Incubate the reaction at room temperature for 2-4 hours or 2-12 hours at 4 degree celcius. The reaction is now ready for purification.

物理性質(zhì)：

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狀態(tài)：淡黃色到黃色固體，或者半固體，取決于分子量。

溶解性：溶于DMSO。

保持條件：-20℃*保存，避光，干燥。

注意事項(xiàng)：取用一定要干燥，避免頻繁的溶解和凍干。

反應(yīng)性：DBCO于含有N3基團(tuán)的反應(yīng)。

技術(shù)指標(biāo)：

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純度（PEG）：90%以上

取代率（DBCO）：90%以上

取代率（FA）：90%以上

分散系數(shù)（PDI）：小于等于1.05

分析：COA、GPC、NMR、MALDI-TOF

Dibenzocyclooctyne-聚乙二醇-葉酸 (DBCO-PEG-FA)

Dibenzocyclooctyne-聚乙二醇-葉酸 (DBCO-PEG-FA)