Dibenzocyclooctyne-聚乙二醇-生物素 (DBCO-PEG-BIOTIN)   

 Dibenzocyclooctyne-聚乙二醇-生物素 (DBCO-PEG-BIOTIN)

???（備注：非常規(guī)分子量：400、600。精確聚合度的小分子定制。）?

結(jié)構(gòu)式：

基本信息：

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中文名稱：Dibenzocyclooctyne-聚乙二醇-生物素 (DBCO-PEG-BIOTIN)

別名：DBCO-PEG-BIOTIN

英文名稱：DBCO-PEG-BIOTIN 

CAS：-

分子量：400、600、1K、2K、3.4K、4K、5K、10K

純度：90%以上

應(yīng)用簡介：

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DBCO (dibenzocycolctyne) PEG Biotin derivatives can go Click Chemistry reaction without a need of any metal catalysts. The strain-promoted 1,3-dipolar cycloaddition of cyclooctynes and azides, also termed as the Cu-free click reaction, is a bioorthogonal reaction that enables the conjugation of two molecules in aqueous solution. DBCO PEG derivatives possess fast kinetics and stability in aqueous buffer. DBCO reagents can be used to label azide-modified biomolecules spontaneous without the need for toxic Cu catalysts. PEGylation can increase solubility and stability and reduce immunogenicity of peptides and proteins. It can also suppress the non-specific binding of charged molecules to the modified surfaces.

Copper-free Click reaction procedures:

Prepare the azide-containing reagents in reaction buffer.

Add DBCO reagents to azide containing reagents. Add 1 mol equivalent of limiting reagent to 1.5~3.0 mol equivalents of abundance reagent.

Incubate the reaction at room temperature for 2~4 hours or 2~12 hours at 4 0C.

Purify conjugates either by dialysis or size-exclusion chromatograph.

物理性質(zhì)：

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狀態(tài)：淡黃色到白色固體，或者半固體，取決于分子量。

溶解性：溶于水，DMSO等溶劑。

保持條件：-20℃*保存，避光，干燥。

注意事項：取用一定要干燥，避免頻繁的溶解和凍干。

反應(yīng)性：DBCO于含有N3基團的反應(yīng)。NHS可以和NH2。

技術(shù)指標(biāo)：

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純度（PEG）：90%以上

取代率（DBCO）：90%以上

取代率（BIOTIN）：90%以上

分散系數(shù)（PDI）：小于等于1.05

分析：COA、GPC、NMR、MALDI-TOF

Dibenzocyclooctyne-聚乙二醇-生物素 (DBCO-PEG-BIOTIN)

Dibenzocyclooctyne-聚乙二醇-生物素 (DBCO-PEG-BIOTIN)