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蛋白磷酸酶1調(diào)控抑制因子亞基1A（PPP1R1A）試劑盒齊一生物科技有限公司新品*，齊一生物科技有限公司長期經(jīng)營大鼠ELISA試劑盒,人elisa試劑盒，小鼠elisa試劑盒等種屬試劑盒，歡迎您訪問我們的，www.qiyibio。。ｃｏｍ全國：歡迎選購！如有什么問題請給我們或者留言，告訴我您的需要，并留下您寶貴的意見和建議

蛋白磷酸酶1調(diào)控抑制因子亞基1A（PPP1R1A）試劑盒ELISA操作步驟
酶聯(lián)免疫吸附實驗材料,試劑,溶液
1．酶標(biāo)板,100μltip頭,1mlEp管,濕盒
2．包被稀釋液（0.05mol/L碳酸鈉-碳酸氫鈉buffer,pH9.6）
碳酸鈉0.15g,碳酸氫鈉0.29g,疊氮鈉0.02g,加雙蒸水100ml,調(diào)pH9.6
3.封閉液(5%小牛血清/PBS溶液)
小牛血清5ml,1*PBS(pH7.4)95ml
4.洗滌液(PBST,pH7.4)
NaCl0.8g,KH2PO40.02g,Na2HPO4.12H2O0.29g,KCl0.02g,Tween200.05ml,疊氮鈉0.01g,加雙蒸水100ml,調(diào)pH7.4
5.樣本稀釋液(PBS,pH7.4)
NaCl0.8g,KH2PO40.02g,Na2HPO4.12H2O0.29g,KCl0.02g,疊氮鈉0.01g,加雙蒸水100ml,調(diào)pH7.4
6.酶標(biāo)第2抗體(羊抗兔)稀釋范圍1:5,000-1:100,000
7.底物液(TMB-*尿素溶液)
①底物液A:TMB20mg,無水乙醇10ml,加雙蒸水100ml.
②底物液B(0.1mol/L檸檬酸-0.2mol/L磷酸二氫鈉緩沖液,pH5.0-5.4)
Na2HPO41.46g,檸檬酸0.933g,0.75%*尿素0.64ml,加三蒸水100ml,調(diào)pH5.0-5.4
③底物A和B按1:1混合即成TMB-*尿素溶液.
8.終止液(2mol/LH2SO4溶液)雙蒸水200ml,濃硫酸34ml,(緩慢滴加并不斷攪拌),加雙蒸水300ml9.0.9%生理鹽水

L-羥脯氨酸 51-35-4
α-蒎烯（95%）2437-95-8
B-蒎烯（95%）127-91-3 
蘋果酸6915-15-7
霹靂蘿芙木堿4382-56-3 
派立辛62499-28-9
天麻素62499-27-8
蒲公英甾醇1059-14-9
秋水仙堿64-86-8
青藤堿115-53-7
鹽酸青藤堿6080-33-7
羥基紅花黃色素A146087-19-6
茄尼醇13190-97-1
秦皮甲素531-75-9
秦皮素574-84-5
秦皮乙素305-01-1
秦皮苷524-30-1
千金子二萜醇34420-19-4
千金藤素481-49-2
千金子甾醇（95%）28649-59-4
千層紙素A480-11-5
青陽參苷元 84745-94-8
10-羥基癸烯酸14113-05-4
烏藥醚內(nèi)酯13476-25-0
去甲異波爾定5890-18-6
奇壬醇52659-56-0
5-羥基色氨酸56-69-9
蘆丁153-18-4
曲克蘆丁7085-55-4
七葉皂苷鈉20977-05-3
七葉皂苷A123748-68-5
 鹽酸去氫駱駝蓬堿343-27-1
去氫駱駝蓬堿442-51-3
5-羥甲基糠醛67-47-0
芹菜素-7-O-β-D-吡喃葡萄糖苷578-74-5
芫花素437-64-9
羥基芫花素20243-59-8
喬松素480-39-7 
榿木酮33457-62-4
 13-去羥基印烏堿
印烏頭4491-19-4
去氫荷包牡丹堿
5-羥基-7,8-二甲氧基黃酮3570-62-5
5-羥基-6,7-二甲氧基黃酮740-33-0
芹菜素-7-O-葡萄糖醛酸苷29741-09-1
芹菜素-7-O-β-D-葡萄糖苷578-74-5
芹菜素-7-O-葡萄糖醛酸苷-6'-乙酯
1-羥基-2,3,4,7-四甲氧基山山酮14103-09-4
芹菜苷26544-34-3
去甲烏藥堿5843-65-2
鹽酸去甲烏藥堿11041-94-4 
羥基酪醇10597-60-1
3,5,6,7,8,3’,4’-七甲氧基黃酮
漆黃素528-48-3
芹菜素520-36-5
肉桂醛104-55-2
肉桂醇104-54-1
肉桂酸621-82-9
擬人參皂苷F1169884-00-0
擬人參皂苷RT598474-78-3
人參皂苷RC11021-14-0
人參皂苷RD52705-93-8
人參皂苷Rb141753-43-9
人參皂苷Rb211021-13-9
人參皂苷Rb368406-26-8
人參皂苷Rg1 22427-39-0
(S型)人參皂苷Rg252286-74-5
 (R型)人參皂苷Rg2
人參皂苷Rg314197-60-5
 20(R)人參皂苷Rg3
Motor, stepper no cable, detector, RG6    9019364    qiagen
Motor-stepper, rotordrive,sourceopt, RG6    9019363    qiagen
FlexiTube GeneSolution siRNA 1 nmol    1027416    qiagen
Photomultiplier with cable, RG6    9019337    qiagen
Rack for Murex Reagents VK54/55,VK    9234679    qiagen
Optic fibre, 550 mm, GSC    9020473    qiagen
Connector, tubing, 1/8 in to 6 mm tubing    9234552    qiagen
AllStars Neg. Control siRNA (20 nmol)    1027281    qiagen
MagAttract Suspension G (1,6/2),KG    1026883    qiagen
DyeEx 2.0 Spin Kit (10)    1026577    qiagen
Frame, cooling, BR3000, 0    9234262    qiagen
8 Wash Head    9233477    qiagen
FastLane Cell cDNA Buffer Set (14x250ml)    1062737    qiagen
Board, Z2 motor    9232909    qiagen
Board, option, non-CE    9232907    qiagen
HBV Quantification Standards    1061052    qiagen
Influenza A RT-PCR REAGENT    1061067    qiagen
Power Supply, RCS1    9016569    qiagen
Transportation box, BioSprint 96    9016537    qiagen
Vacuum Tray, plete, BR8 RD, UK    9016463    qiagen
BioRobot MDx Performance Upgrade Kit    9016267    qiagen
Temp. Sensor, PyroQ96    9019184    qiagen
Opto - plate lock open, PyroQ96MD    9019178    qiagen
Label, cover, main, RG6    9019879    qiagen
Cover, main, red, RG6    9019876    qiagen
Fuse, 4A, 250VAC, V2, QX    9019762    qiagen
Motor, Z, V2, QX    9019733    qiagen
Transport lock, V2, QX    9019726    qiagen
Cable set, cooling system, 200    9013404    qiagen
Rect. sealing cap, clear, incl. clips    9238803    qiagen
Base Plate VPT, PyroQ96VW    9019653    qiagen
Tip ejector, Cx2    9019626    qiagen
Pins, locating, Mix and Reagent set, CG1    9019603    qiagen
Lead, vac trap sensor, CX1    9019598    qiagen
Belt, X-axis assembly, CQx    9019573    qiagen
Clamp, cable, 3 wires 2 tabs, Cxx    9019546    qiagen
PCB, USB, QGY    9019533    qiagen
RISER VACUUM 16.25MM    9019518    qiagen
miRNeasy Mini Kit (50)    217004    qiagen
QuantiTect SYBR Green RT-PCR Kit (200)    204243    qiagen
Heating block assy    9019439    qiagen
Gasket, lower chamber, RG3    9019425    qiagen
Box, shipping with foam packing, RG3    9019416    qiagen
Connector, COM incl. int. cable, RG3    9019401    qiagen
Switch, mains voltage selector, RG6    9019360    qiagen
Flexitube siRNA 5 nmol    1027417    qiagen
LED, green (530), RG6    9019339    qiagen
EPROM, IC Motherbrd, state FW, RG6    9019331    qiagen
Linear guide - X, PyroQ24    9019237    qiagen
X-Assembly Turntable Card    9234673    qiagen
Cassette, HSPS, click'n'go    9234659    qiagen
Buffer AVE (Elution Buffer) (125/125),KG    1026956    qiagen
RNeasy 96 Plates (12)    1026905    qiagen
Probe, ceramic coat., 0.4mm, 158mm    9233674    qiagen
Mousepad BR 01/10 (WW)    9233630    qiagen
Motor - Gripper, PyroQ96MD    9019209    qiagen
Damper kit - Robot, PyroQ96MD    9019197    qiagen
Buffer AW2 with Azid (127/500),KG    1020955    qiagen
HBV PCR REAGENT III    1062791    qiagen
Spritzenpumpe mMBP 2021C P7xxx    9232903    qiagen
PG Influenza A RT-PCR Assay (48) Z    1061065    qiagen
Valve, release, auto vac, BR8000    9232900    qiagen
Software Package LiquiChip Analyzer    9016710    qiagen
Pipetting head, 8 ch. , CX1    9019891    qiagen
Lid with LED, red, RG6    9019881    qiagen
36-well High Profile Locking Ring    9019857    qiagen
IPC Hard Drive PCA6751, PyroQ96MD    9019837    qiagen
Notebook_V3 W7_32bit    9022832    qiagen
Tubing, syringe to connector, BR3000    9013703    qiagen
Membrane, Simritz (20)    9013485    qiagen
Round sealing cap, clear    9238805    qiagen
Cable, UV lamp volt. conv., long, EZ1xl    9019637    qiagen
Cover, plexiglas, 8 ch., CX1    9019590    qiagen
Clamp, cable 2 wires 2 tabs, Cxx    9019565    qiagen
Bow spring, table rail, Cxx    9019543    qiagen
CHAMBER MAIN VAC STATION 1820    9019510    qiagen
PLATE SEPARATOR 48 WELL HIGH    9019505    qiagen
QuantiFast Probe PCR Kit (2000)    204256    qiagen