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現(xiàn)貨人Ⅲ型前膠原肽（PⅢNP）ELISA檢測試劑盒齊一生物的ELISA試劑盒是您的*選擇:每個(gè)試劑盒都經(jīng)過嚴(yán)格的質(zhì)檢控制，包括：板內(nèi)、板間實(shí)驗(yàn)精度，靈敏度，回收率，穩(wěn)定性等指標(biāo)的測定。我們提供的96孔板已經(jīng)預(yù)包被好捕獲抗體，三步法檢測體系簡單而直接，可根據(jù)450 nm波長的讀值迅速計(jì)算定量結(jié)果。試劑盒中提供的檢測抗體能夠識別全長靶蛋白的抗原決定簇以及它們的三維結(jié)構(gòu)，使您的檢測結(jié)果更穩(wěn)定可靠

現(xiàn)貨人Ⅲ型前膠原肽（PⅢNP）ELISA檢測試劑盒ELISA試劑盒操作步驟
ELISA試劑盒使用前,將所有試劑充分混勻.不要使液體產(chǎn)生大量的泡沫,以免加樣時(shí)加入大量的氣泡,產(chǎn)生加樣上的誤差.
根據(jù)待測樣品數(shù)量加上標(biāo)準(zhǔn)品的數(shù)量決定所需的板條數(shù).每個(gè)標(biāo)準(zhǔn)品和空白孔建議做復(fù)孔.每個(gè)樣品根據(jù)自己的數(shù)量來定,能使用復(fù)孔的盡量做復(fù)孔.標(biāo)本用標(biāo)本稀釋液1：1稀釋后加入50ul于反應(yīng)孔內(nèi).
加入稀釋好后的標(biāo)準(zhǔn)品50ul于反應(yīng)孔、加入待測樣品50ul于反應(yīng)孔內(nèi).立即加入50ul的生物素標(biāo)記的抗體.蓋上膜板,輕輕振蕩混勻,37℃溫育1小時(shí).
甩去孔內(nèi)液體,每孔加滿洗滌液,振蕩30秒,甩去洗滌液,用吸水紙拍干.重復(fù)此操作3次.如果用洗板機(jī)洗滌,洗滌次數(shù)增加一次.
每孔加入80ul的親和鏈酶素-HRP,輕輕振蕩混勻,37℃溫育30分鐘.
甩去孔內(nèi)液體,每孔加滿洗滌液,振蕩30秒,甩去洗滌液,用吸水紙拍干.重復(fù)此操作3次.如果用洗板機(jī)洗滌,洗滌次數(shù)增加一次.
每孔加入底物A、B各50ul,輕輕振蕩混勻,37℃溫育10分鐘.避免光照.
取出酶標(biāo)板,迅速加入50ul終止液,加入終止液后應(yīng)立即測定結(jié)果.
在450nm波長處測定各孔的OD值.
ELISA操作步驟
酶聯(lián)免疫吸附實(shí)驗(yàn)材料,試劑,溶液
1．酶標(biāo)板,100μltip頭,1mlEp管,濕盒
2．包被稀釋液（0.05mol/L碳酸鈉-碳酸氫鈉buffer,pH9.6）
碳酸鈉0.15g,碳酸氫鈉0.29g,疊氮鈉0.02g,加雙蒸水100ml,調(diào)pH9.6
3.封閉液(5%小牛血清/PBS溶液)
小牛血清5ml,1*PBS(pH7.4)95ml
4.洗滌液(PBST,pH7.4)
NaCl0.8g,KH2PO40.02g,Na2HPO4.12H2O0.29g,KCl0.02g,Tween200.05ml,疊氮鈉0.01g,加雙蒸水100ml,調(diào)pH7.4
5.樣本稀釋液(PBS,pH7.4)
NaCl0.8g,KH2PO40.02g,Na2HPO4.12H2O0.29g,KCl0.02g,疊氮鈉0.01g,加雙蒸水100ml,調(diào)pH7.4
6.酶標(biāo)第2抗體(羊抗兔)稀釋范圍1:5,000-1:100,000
7.底物液(TMB-*尿素溶液)
①底物液A:TMB20mg,無水乙醇10ml,加雙蒸水100ml.
②底物液B(0.1mol/L檸檬酸-0.2mol/L磷酸二氫鈉緩沖液,pH5.0-5.4)
Na2HPO41.46g,檸檬酸0.933g,0.75%*尿素0.64ml,加三蒸水100ml,調(diào)pH5.0-5.4
③底物A和B按1:1混合即成TMB-*尿素溶液.
8.終止液(2mol/LH2SO4溶液)雙蒸水200ml,濃硫酸34ml,(緩慢滴加并不斷攪拌),加雙蒸水300ml
9.0.9%生理鹽水

130718    NeXtal Tubes PACT Suite
19088    Reagent DX
218411    Human miScript Primer Assay Set VXX.X
9019899    Cable - Ground, PyroQ96ID
9019003    Hinge, for door, EZ1xl
9019004    Grid, UV lamp, EZ1xl
9019021    Damper, door, EZ1xl
9019759    Power supply, main, 24V, V2, QX
950154    Wash Reagent (DXW) (650 ml)
9019537    Plate Stacker A/B RD, RCS
9021031    Frame, transfer module, Qsym
337042    qBiomarker Assay PreAMP Mix
51161    QIAamp 96 DNA Blood Kit (4)    For 4 x 96 DNA preps: 4 QIAamp 96 Plates, QIAGEN Protease, Reagents, Buffers, Lysis Blocks, Tape Pads, Collection Vessels
75162    RNeasy Maxi Kit (12)    12 RNeasy Maxi Spin Columns, Collection Tubes (50 ml), RNase-free Reagents and Buffers
120016    QIAquick 96 Plates (24)    Individual QIAquick 96 plates and handbook. No buffers or reagents.
203443    HotStarTaq Master Mix Kit (250)    "3 x 0.85 ml HotStarTaq Master Mix* containing 250 units HotStarTaq DNA Polymerase total, 2 x 1.7 ml distilled water

- premixed solution for high PCR specificity
- HotStarTaq Master Mix, HotStarTaq DNA Polymerase, several kit sizes from 250 u up to 2500 u available"
301108    PolyFect Transfection Reagent (100 ml)    For 2500-6500 transfections in 60 mm dishes or 5000-10,000 transfections in 6-well plates.
231222    QIAGEN PCR Cloning plus Kit (10)    For 10 reactions: 2x Ligation Master Mix (50 ul), pDrive Cloning Vector (0.5 ug), distilled water (1.7 ml), QIAGEN EZ Competent Cells (10 tubes, 50 ul each), SOC medium (2 x 1.9 ml)
9015638    AC fuse, 115 V, 250 8A, M48
132568    NeXtal Stock Methoxy-PEG 5000 (50)    30% w/v Methoxypolyethylene glycol 5000 - 50mL (4 x 12,5 ml tube)
9019577    Opto switch, Z-axis, 1 ch. , Cx2
9019594    Belt, Z-axis gearbox (black), 8 ch., Cx8
9019068    PyroMark CpG SW 1.0 (5)    "Software for PyroMark Q96 ID and PyroMark Q96 MD for analysis and quantification of CpG methylation in epigenetic studies. 
Five additional software licenses for PyroMark CpG SW 1.0"
9019086    PyroMark Q96 MD Software (5)    "For use with PyroMark Q96 MD instrument.Software for setting up a run and analyzing mutations.

5 licenses"
9019658    O rings - VPT Fast connector, PyroVW
972012    PyroMark Q96 CpG p16 (96)    For quantification of methylation level in p16
979003    PyroMark Q96 Sample Prep Tool Cover (10)    Consumables for use with magnetic tool for isolation of single stranded DNA.
979008    PyroMark Wash Buffer (conc., 200 ml)    For washing and neutralizing the immobilized DNA.
950140    Binding Additive (DX) (4.0 g)
9019803    Upgrade Package 1, RCS
130803    NeXtal CubicPhase 1w Mo μplate (10)
9243044    Set, brackets, transparent panel, Qsym
283615    virotype PEDV/TGEV RT-PCR Rgt (96)
52906    QIAamp Viral RNA Mini Kit (250)    For 250 RNA preps: 250 QIAamp Mini Spin Columns, Carrier RNA, Collection Tubes (2 ml), RNase-free Buffers
63183    DyeEx 96 Kit (24)    24 DyeEx 96 Plates; 4 Collection Plates, 48-Well
69582    DNeasy 96 Blood & Tissue Kit (12)    For 12 x 96 DNA minipreps: 12 DNeasy 96 Plates, QIAGEN Proteinase K, Buffers, Square-Well Blocks, AirPore Tape Sheets, Collection Microtubes (1.2 ml), Caps, 96-well Plate Registers
70042    Oligotex mRNA Midi Kit    For 12 mRNA midipreps: 700 ul Oligotex Suspension, Small Spin Columns, Collection Tubes (1.5 ml), RNase-free Reagents and Buffers
79523    Small Spin Columns    24 RNase-free spin columns for Oligotex spin procedures
301109    PolyFect Transfection Reagent (10x100ml)
9239361    PCB-micro-fuse T 6.3A, 250V, EZ1
132632    NeXtal Stock Potassium formate (50)    10M Potassium formate - 50mL (4 x 12,5 ml tube)
9019610    Cover, Pipettor, 1 ch. (white), CAS
9019624    PCB, lge breakout, 12 core to rev 3, Cx2
9019067    PyroMark CpG SW 1.0    Software for PyroMark Q96 ID and PyroMark Q96 MD for analysis and quantification of CpG methylation in epigenetic studies.
9019087    PyroMark IdentiFire SW 1.0    Software for sequence-based species identification by paring and matching Pyrosequencing data to a local database.
972052    PyroMark Q96 CpG Prader Willi (96)    For quantification of methylation level in  Prader-Willi and Angelman Syndromes
979002    PyroMark Q96 Plate Low (100)    For sample analysis of DNA template on ID.
979004    PyroMark Q96 Cartridge (3)    For delivery of nucleotides and reagents for use in PyroMark Q96 ID.
9001513    PyroMark Q24 MDx    For methylation and mutation analysis using Pyrosequencing technology in 24 well CE-marked.
990610    Filter-Tips, OnCor C, 200 μl (960)
9017842    Tool, gauge, 30 mm, calibration, QIAC
9020806    Rotor Timing Calibration Dye, RGQ
331455    miScript Microfluidics PreAMP Kit (96)
381566    Investigator ESSplex SE GO! Kit (200)
9022654    Transfer Carriage QIAplate, QXT
16191    QIAwell 96 Ultra Plasmid Kit (4)    For 4 x 96 ultrapure plasmid minipreps, 4 each: QIAfilter 96, QIAwell 96, and QIAprep 96 Plates; Reagents, Buffers, Collection Microtubes (1.2 ml), Caps
19046    Plasmid Buffer Set    Buffers P1, P2, P3, QBT, QC, QF, RNase A; for 100 plasmid mini-, 25 midi-, or 10 maxipreps
19201    Collection Tubes (2-ml)    1000 Collection Tubes (2 ml)
9015862    EZ1 DNA PRFN Card    Pre-programmed card for BioRobot EZ1 paraffin section protocols
67163    MagAttract 96 DNA Plant Core Kit (24)    MagAttract Suspension A and buffers for 24 x 96 preps
1030483    Elution Microtubes CL, PAX, FLU
9232687    Hood f. Tip Disposal Station 2.01
9015972    Tube Holder, STS MDx, 15 mm (12)
158926    Cell Suspension Solution (125 ml)
159992    Generation DNA Purif. Soln 1 (1000 ml)
201913    dNTP Set, PCR Grade 100mM, (4x250μl)    dNTP Set is a plete set of individual, highly pure dNTPs: 100 mM each dATP, dCTP, dGTP, dTTP for 2500 x 50ul PCR reactions
12945    QIAGEN Plasmid Plus Midi Kit (100)    "100 QIAGEN Plasmid Plus Midi Columns, Extender Tubes, Reagents, Buffers, 100 QIAfilter Midi Cartridges

QIAGEN Plasmid Plus Kits enable ultrafast, large-scale plasmid purification of pure plasmid DNA. The use of a vacuum manifold allows purification of up to 12 (Mega and Giga) or up to 24 (Midi and Maxi) samples in parallel with low hands-on needs. Low elution volumes yield highly concentrated plasmid DNA for direct use without ethanol precipitation. QIAGEN Plasmid Plus Kits also feature a novel wash buffer for endotoxin reduction."
9019715    PyroMark Q24 User Manual
9019861    Rotor Locking Nut Set
9019890    Foam insert, rotor securing, QIAC
336391    SureSilencing shRNA Control
330522    RT2 SYBR Green ROX qPCR Mastermix (12)
330411    RT2 HT First Strand Kit (96)
336161    Multi-Analyte  ELISArray Kit
9020716    Optoswitch, X axis, C34
9020712    Rail, x axis, C34
9242544    Power Input Module, Eppendorf, AP196/98
9020713    Rail guide, x axis, C34
9020911    Foot assembly, SMC, QGX
9020679    Rail, left, CX1
9022916    Gas press.spring conversion kit QG/QXT
9012771    Liquid Handling Kit Plato 7382/7182
9013150    BGR Tischabstützung  Digene
9013205    BGR Labhand li+re Digene
9014878    Card, x-main, shaker
202605    HotStar HiFidelity Polymerase Kit (1000)    2 x 500 units HotStar HiFidelity DNA Polymerase, 5x PCR Buffer containing dNTPs, 5x Q-Solution, 25 mM MgSO4, RNase free water
9019368    Damper, lid, RG6
9019386    Photo Multiplier Tube with cable, RG3
9019393    Solenoid, cooling with flap, RG3
9019417    Fan, lid, RG3
9019424    Wiring, lower heater, RG3
9019446    Memory card 32MB SDl w/S/W
9019451    Switch incl. 2K2 resistor, P/R, HS
9019473    Loading guide, 52 well sharkstooth, GSC
9019505    PLATE SEPARATOR 48 WELL HIGH
9019510    CHAMBER MAIN VAC STATION 1820
9019543    Bow spring, table rail, Cxx
9019565    Clamp, cable 2 wires 2 tabs, Cxx
9242545    Quick Disconnect O-ring set, LC
9242337    Tool, calibration, AS, Qsym
9018092    Cooling adapter, RG strip tubes 72, Qsym
9020390    Kit, fuse AS, Qsym
4870004    PG Influenza A RT-PCR Kit (48) Z
9022321    RS-232 Cable (2), careHPV
273505    pigtype Trichinella Ab (20)
273401    pigtype Toxoplasma Ab (1)
270043    cattletype BHV1 gB Ab (5)
288103    bactotype Mycoplasma Mg/Ms PCR Kit (24)
9021234    Vacuum Pump (100 V), PyroQ24VW
9011151    Valve, dilutor, 4-port
204443    QuantiTect Probe RT-PCR Kit (200)    For 200 x 50 ul reactions: 3 x 1.7 ml QuantiTect Probe RT-PCR Master Mix,* 100 ul QuantiTect RT Mix, 2 x 2.0 ml RNase-free water
9011243    Holder, bar-code camera, STS, BR3000
9011501    Coupling, Z motor, buffer track, BR8000
9012390    Wash station, 4-probes v2.01
9012671    Cable, PCON98 to release valve, BR8000
4500265    artus HSV-1/2 RG PCR Kit (96) CE
130703    NeXtal Tubes Cryos Suite
132101    NeXtal DWBlock Adhesive Foils
9019509    Pinch Valve, C18
9019542    Gas Strut, lid, 145 N, CQx
9019563    Bracket, mounting Y-axis, Cxx
9020392    PCB, connect drawer, chassis AS, Qsym
9020633    Pos. aid (1), waste box, Cabinet Qsym
9020252    UPG Waste bag holder, Qsym
9242546    Probe Holder Assembly, LX200, LC
4872004    PG AIV-H5 RT-PCR Kit (48)  Z
1072538    HotStarTaq DNA Polymerase (5x5000)
9022474    Shipment Box, careHPV
619024    careBrush, CE
271906    cattletype Milk Prep Kit (50)
280437    virotype BTV RT-PCR Kit (480)