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Mouse Anti-EGF（Epidermal growth factor）to human抗體說明書

Catalog Number : Rs-2008R

Quantity size : 0.2ml （dilute with pH 7.4 0.01 M PBS or antibody diluent ）

Background: Epidermal growth factor （EGF） has a profound effect on the differentiation of specific cells in vivo and is a potent mitogenic factor for a variety of cultured cells. The EGF precursor is believed to exist as a membrane bound molecule which is proteolytically cleaved to generate the 53 amino acid peptide hormone that stimulates cells to divide. EGF exerts its actions by binding to the EGFR, a 170 kDa protein.

Specificity :  · anti- EGF is a Mouse monoclonal antibody, unconjugated

· specific for EGF of human

· epitope mapping in the middle of EGF of human

· Protein G affinity chromatography purification, purity :>96%

· Isotype: IgG

· use for western blotting, elisa, immunoprecipitation and

immunohistochemistry

· mol wt: 161kDa

Application: · Western blotting 1:100-500

· Immunohistochemistry 1:100-500

· ELISA 1:500-1000

· Optimal working dilutions must be determined by the end user.

Storage: Store at –20 ºC for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20ºC. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody, the antibody is stable for at least two weeks at 2-4 ºC.

Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.

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