蛋白名稱:IL6R人重組蛋白,10398-H08H
蛋白構建:A DNA sequence encoding the extracellular domain of human IL6R (NP_000556.1) corresponding to amino acid (Met1-Pro365) was expressed with a C-terminal polyhistidine tag.
表達宿主:HEK293 Cells
蛋白純度:≥ 90 % as determined by SDS-PAGE ≥ 95 % as determined by SEC-HPLC.
蛋白活性:
1. Immobilized Recombinant Human IL6 / Interleukin-6 Protein (Cat: 10395-HNAE) at 2μg/mL (100μL/well) can bind Recombinant Human IL6R / CD126 Protein (His Tag) (Cat: 10398-H08H),the EC50 is 8-24 ng/mL.
2. Measured by its ability to enhance the IL6 activity on M1 mouse myeloid leukemia cells. The ED50 for this effect is typically 20-80 ng/mL.
3. Using the Octet RED System, the affinity constant (Kd) of IL-6R Protein, Human, Recombinant (His Tag) (Cat. 10398-H08H) bound Anti-IL6R Antibody (Cat. 10398-MM08) was 0.1 nM.
4. Loaded Recombinant Human IL-6R Protein, His Tag (Cat. No. 10398-H08H) on HIS1K Biosensor, can bind Recombinant Human IL-6 Protein (Cat. No. 10395-HNAE) with an affinity constant of 75.9 nM as determined in BLI assay (Sartorius Octet RED384) (Routinely tested).
蛋白內(nèi)毒素:< 1.0 EU per μg of the protein as determined by the LAL method
預測N端:Leu 20
蛋白分子量:The soluble form of recombinant human IL6R consists of 357 amino acids and has a predicted molecular mass of 40 kDa. In SDS-PAGE under reducing conditions, it migrates with an apparent molecular mass of 60-65 kDa due to glycosylation.
蛋白NP號:NP_000556.1
蛋白氨基酸序列:Met1-Pro365
蛋白標簽:C-His
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義翹神州研發(fā)團隊擁有多年的重組蛋白研發(fā)經(jīng)驗。多年來,我們的團隊已開發(fā)出超過9300余種重組蛋白,涵蓋免疫檢查點、抗體藥物靶點、CAR-T細胞治療靶點、Fc受體、流感病毒蛋白以及細胞因子等熱門研究領域。義翹神州重組蛋白具有高純度、高生物活性和低內(nèi)毒素水平等特性,可為生物藥靶點發(fā)現(xiàn)、蛋白質(zhì)結構和功能分析、細胞療法和重組酶酶學性質(zhì)等科學研究提供支持。
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