CAS號	434-13-9	產(chǎn)地	國產(chǎn)
規(guī)格	500mg	級別	化工級
證書	ISO系列證書

Lithocholic acid (Synonyms: 石膽酸; 3α-Hydroxy-5β-cholanic acid)

Lithocholic acid是有毒的次級膽汁酸，能促使肝內膽汁淤積，能促進腫瘤發(fā)生。

生物活性

Lithocholic acid is a toxic secondary bile acid, causes intrahepatic cholestasis, has tumor-promoting activity. Target: Others Lithocholic acid has been used in a study to assess cholestasis and its action on several organs and tissues in rats. It has also been used in a study to investigate the regulation of hepatic phospholipid and bile acid homeostasis through SMAD3 activation by TGFβ. It has been implicated in human and experimental animal carcinogenesis. Preliminary in vitro research suggests that LCA selectively kills neuroblastoma

IC₅₀ & Target

Microbial Metabolite Human Endogenous Metabolite

分子量：376.57

Formula：C₂₄H₄₀O₃

CAS 號：434-13-9

中文名稱：石膽酸

結構分類：

Steroids

運輸條件：Room temperature in continental US; may vary elsewhere.

儲存方式

Powder	-20°C	3 years
	4°C	2 years
In solvent	-80°C	6 months
	-20°C	1 month

溶解性數(shù)據(jù)

In Vitro:

DMSO : 250 mg/mL (663.89 mM; Need ultrasonic)

Ethanol : 10 mg/mL (26.56 mM; ultrasonic and warming and heat to 60°C)

配制儲備液

濃度溶劑體積質量	1 mg	5 mg	10 mg

1 mM	2.6555 mL	13.2777 mL	26.5555 mL
5 mM	0.5311 mL	2.6555 mL	5.3111 mL
10 mM	0.2656 mL	1.3278 mL	2.6555 mL

請根據(jù)產(chǎn)品在不同溶劑中的溶解度選擇合適的溶劑配制儲備液；一旦配成溶液，請分裝保存，避免反復凍融造成的產(chǎn)品失效。
儲備液的保存方式和期限：-80°C, 6 months; -20°C, 1 month。-80°C 儲存時，請在 6 個月內使用，-20°C 儲存時，請在 1 個月內使用。

In Vivo:

以下溶解方案都請先按照 In Vitro 方式配制澄清的儲備液，再依次添加助溶劑：

——為保證實驗結果的可靠性，澄清的儲備液可以根據(jù)儲存條件，適當保存；體內實驗的工作液，建議您現(xiàn)用現(xiàn)配，當天使用；以下溶劑前顯示的百
分比是指該溶劑在您配制終溶液中的體積占比；如在配制過程中出現(xiàn)沉淀、析出現(xiàn)象，可以通過加熱和/或超聲的方式助溶

1.

請依序添加每種溶劑： 10% DMSO 90% (20% SBE-β-CD in saline)
Solubility: ≥ 2.08 mg/mL (5.52 mM); Clear solution
2.

請依序添加每種溶劑： 10% DMSO 90% corn oil
Solubility: ≥ 2.08 mg/mL (5.52 mM); Clear solution
3.

請依序添加每種溶劑： 10% EtOH 40% PEG300 5% Tween-80 45% saline
Solubility: ≥ 1 mg/mL (2.66 mM); Clear solution
4.

請依序添加每種溶劑： 10% EtOH 90% (20% SBE-β-CD in saline)
Solubility: ≥ 1 mg/mL (2.66 mM); Clear solution
5.

請依序添加每種溶劑： 10% EtOH 90% corn oil
Solubility: ≥ 1 mg/mL (2.66 mM); Clear solution

參考文獻

[1]. Jenkins, D.J., et al., Effect on blood lipids of very high intakes of fiber in diets low in saturated fat and cholesterol. N Engl J Med, 1993. 329(1): p. 21-6.

[2]. Yang R, et al. Metabolomic analysis of cholestatic liver damage in mice. Food Chem Toxicol. 2018 Jul 14;120:253-260.

[3]. Goldberg, A.A., et al., Lithocholic bile acid selectively kills neuroblastoma cells, while sparing normal neuronal cells. Oncotarget, 2011. 2(10): p. 761-82.

[4]. Matsubara, T., et al., TGF-beta-SMAD3 signaling mediates hepatic bile acid and phospholipid metabolism following lithocholic acid-induced liver injury. J Lipid Res, 2012. 53(12): p. 2698-707.

注：產(chǎn)品僅用于科研