waters 蛋白質(zhì)反相色譜柱

反相色譜

waters 蛋白質(zhì)反相色譜柱是一種基于蛋白質(zhì)在溶液中的相對(duì)疏水性的分離技術(shù)，分析時(shí)使用裝填有短鏈鍵合相的大孔徑顆粒（例如，300?）的色譜柱。如果使用離子對(duì)試劑（例如0.1% TFA）來zui大限度減少不良離子干擾，通常利用逐漸升高的有機(jī)溶劑濃度梯度來控制分離。一般來說，洗脫順序取決于蛋白質(zhì)或蛋白質(zhì)亞基的疏水性，疏水性zui弱的蛋白質(zhì)將首先洗脫出來。此外，顆粒組成（硅膠vs雜化顆粒）、孔徑、鍵合相類型和密度，以及分離條件（例如梯度持續(xù)時(shí)間、分離溫度、流速）等等對(duì)于實(shí)現(xiàn)滿足應(yīng)用要求的分離而言也非常重要。

BEH顆粒技術(shù)

專為蛋白質(zhì)分離設(shè)計(jì)

分離大分子生物化合物的難度較大。20多年來，沃特世反相色譜柱在蛋白質(zhì)分離方面的性能優(yōu)勢為色譜工作者提供了有力支持。面對(duì)棘手的蛋白質(zhì)分離難題，沃特世亞乙基橋雜化(BEH)顆粒技術(shù)有助于用戶突破使用*硅膠基質(zhì)填料色譜柱時(shí)的性能局限。

• 300 ?孔徑

• C4鍵合相

• zui大限度減少不良次級(jí)相互作用

• zui大限度減少高溫分離時(shí)的可檢出殘留

• 可重現(xiàn)的蛋白質(zhì)分離

• 采用成熟的*鍵合工藝

• 在低pH和高溫條件下穩(wěn)定

• 采用多種蛋白質(zhì)混合物進(jìn)行質(zhì)控測試

• 出色的批次間重現(xiàn)性

• 提供適用于UPLC和微升級(jí)UPLC應(yīng)用的1.7 μm顆粒規(guī)格

• 使用納流和微流LC-MS進(jìn)行蛋白質(zhì)分離

  150 μm iKey分離裝置的“即插即用”式設(shè)計(jì)和無接頭流路連接大大簡化了采用微流LC-MS的蛋白質(zhì)分析方法。與1 mm內(nèi)徑的UPLC相比，iKey將分析靈敏度提高了多達(dá)40倍。

• CQUITY UPLC M-Class色譜柱專為低擴(kuò)散納流LC而設(shè)計(jì)，適用于納流LC-MS蛋白質(zhì)分析。納升級(jí)至微升級(jí)LC/MS ACQUITY UPLC M-Class色譜柱和捕集柱可在15000 psi的UPLC條件下進(jìn)行納升級(jí)和微升級(jí)分離，充分利用亞2 μm顆粒技術(shù)的分離能力。

• BioSuite pPhenyl反相色譜(RPC) HPLC色譜柱

  BioSuite RPC色譜柱產(chǎn)品中裝填有與pH穩(wěn)定的甲基丙烯酸酯類聚合物樹脂鍵合的苯基(pPhenyl)填料。pPhenyl基質(zhì)的孔徑為1000 ?，可分析分子量達(dá)5 000 000道爾頓的蛋白質(zhì)。

  pPhenyl RPC填料提供裝填于5 x 150 mm色譜柱的規(guī)格，適用于“實(shí)驗(yàn)室規(guī)模”分離；還提供裝填于2.0 x 75 mm色譜柱的規(guī)格，適用于窄徑HPLC和LC-MS應(yīng)用。

• Symmetry300 C4 HPLC和UHPLC色譜柱

  Symmetry300 C4是采用超純有機(jī)試劑合成的*硅膠基質(zhì)材料，純度*且硅醇活性極低，對(duì)肽和蛋白質(zhì)的分離和回收效果較佳。

• 300 ?孔徑，適用于肽和蛋白質(zhì)應(yīng)用

• *封端，zui大限度減少不良次級(jí)相互作用

• 相較于Waters BEH C4, 300 ?雜化填料，為分離選擇性帶來更多選擇

• 采用肽標(biāo)準(zhǔn)品進(jìn)行QC測試，可確保出色的批次間*性

• Delta-Pak色譜柱

• Delta-Pak HPLC色譜柱基于高度穩(wěn)定、鍵合、封端的球形5 μm或15 μm 100％硅膠基質(zhì)顆粒。該系列色譜柱有100 ?和300 ?兩種孔徑 - 填充C4或C18鍵合相，可滿足不同的選擇性和保留性需求。提供不同的15 μm小柱和色譜柱配置，能夠?qū)崿F(xiàn)*且可預(yù)測的毫克級(jí)到克級(jí)純化放大。

• 訂貨信息

  貨號(hào)	描述
  186007567	ACQUITY UPLC M-Class Protein BEH C4 Column, 300?, 1.7 µm, 300 µm X 50 mm, 1/pkg
  186007569	ACQUITY UPLC M-Class Protein BEH C4 Column, 300?, 1.7 µm, 300 µm X 150 mm, 1/pkg
  WAT046885	DeltaPak C4 Guard Column, 300?, 5 µm, 3.9 mm X 20 mm, 2/pkg
  186007495	ACQUITY UPLC M-Class Protein BEH C4 Column, 300?, 1.7 µm, 150 µm X 100 mm, 1/pkg
  WAT011812	DeltaPak C4 Column, 300?, 15 µm, 3.9 mm X 300 mm, 1/pkg
  WAT036865	DeltaPak C4 Guard-Pak Insert, 300?, 5 µm, 10/pkg
  186007493	ACQUITY UPLC M-Class Protein BEH C4 Column, 300?, 1.7 µm, 75 µm X 100 mm, 1/pkg
  186007494	ACQUITY UPLC M-Class Protein BEH C4 Column, 300?, 1.7 µm, 100 µm X 100 mm, 1/pkg
  186005589	ACQUITY UPLC Protein BEH C4 Column, 300?, 1.7 µm, 1 mm X 50 mm, 1/pkg
  186004899	ACQUITY UPLC Protein BEH C4 Method Validation Kit, 300?, 1.7 µm, 2.1 mm X 100 mm, 3/pkg
  186006549	ACQUITY UPLC Protein BEH C4 Method Validation Kit, 300?, 1.7 µm, 2.1 mm X 150 mm, 3/pkg
  186002156	BioSuite Phenyl Column, 1000?, 10 µm, 2 mm X 75 mm, 1/pkg
  186005590	ACQUITY UPLC Protein BEH C4 Column, 300?, 1.7 µm, 1 mm X 100 mm, 1/pkg
  186005591	ACQUITY UPLC Protein BEH C4 Column, 300?, 1.7 µm, 1 mm X 150 mm, 1/pkg
  WAT011794	DeltaPak C4 Column, 300?, 5 µm, 3.9 mm X 150 mm, 1/pkg
  186004497	ACQUITY UPLC Protein BEH C4 Column, 300?, 1.7 µm, 2.1 mm X 150 mm, 1/pkg
  186004623	ACQUITY UPLC Protein BEH C4 VanGuard Pre-column, 300?, 1.7 µm, 2.1 mm X 5 mm, 3/pkg
  186004496	ACQUITY UPLC Protein BEH C4 Column, 300?, 1.7 µm, 2.1 mm X 100 mm, 1/pkg
  WAT011796	DeltaPak C4 Column, 100?, 5 µm, 3.9 mm X 150 mm, 1/pkg
  186004495	ACQUITY UPLC Protein BEH C4 Column, 300?, 1.7 µm, 2.1 mm X 50 mm, 1/pkg
  WAT011807	DeltaPak C4 Column, 100?, 15 µm, 3.9 mm X 300 mm, 1/pkg
  186002157	BioSuite Phenyl Column, 1000?, 10 µm, 4.6 mm X 75 mm, 1/pkg
  WAT046875	DeltaPak C4 Guard Column, 100?, 5 µm, 3.9 mm X 20 mm, 2/pkg
  186002158	BioSuite Phenyl Column, 1000?, 13 µm, 21.5 mm X 150 mm, 1/pkg
  WAT011813	DeltaPak C4 Prep Column, 300?, 15 µm, 7.8 mm X 300 mm, 1/pkg
  WAT011814	DeltaPak C4 Prep Column, 300?, 15 µm, 19 mm X 300 mm, 1/pkg
  186000277	Symmetry C4 Column, 300?, 3.5 µm, 2.1 mm X 50 mm, 1/pkg
  186000278	Symmetry C4 Column, 300?, 3.5 µm, 2.1 mm X 100 mm, 1/pkg
  186000279	Symmetry C4 Column, 300?, 3.5 µm, 2.1 mm X 150 mm, 1/pkg
  WAT011810	DeltaPak C4 Prep Column, 100?, 15 µm, 30 mm X 300 mm, 1/pkg
  WAT011669	DeltaPak C4 Radial-Pak Cartridge, 300?, 15 µm, 47 mm X 300 mm, 1/pkg
  WAT037700	DeltaPak C4 Radial-Pak Cartridge, 300?, 15 µm, 40 mm X 100 mm, 1/pkg
  WAT025848	DeltaPak C4 Radial-Pak Cartridge, 100?, 15 µm, 8.0 mm X 100 mm, 1/pkg
  186000286	Symmetry C4 Column, 300?, 5 µm, 3.9 mm X 150 mm, 1/pkg
  186000285	Symmetry C4 Column, 300?, 5 µm, 2.1 mm X 150 mm, 1/pkg
  WAT037851	DeltaPak C4 Radial-Pak Guard, 300?, 15 µm, 40 mm X 10 mm, 2/pkg
  WAT038524	DeltaPak C4 Radial-Pak Guard, 100?, 15 µm, 25 mm X 10 mm, 2/pkg
  186000283	Symmetry C4 Column, 300?, 3.5 µm, 4.6 mm X 150 mm, 1/pkg
  WAT038526	DeltaPak C4 Radial-Pak Guard, 300?, 15 µm, 25 mm X 10 mm, 2/pkg
  186000282	Symmetry C4 Column, 300?, 3.5 µm, 4.6 mm X 100 mm, 1/pkg
  186000281	Symmetry C4 Column, 300?, 3.5 µm, 4.6 mm X 75 mm, 1/pkg
  186000280	Symmetry C4 Column, 300?, 3.5 µm, 4.6 mm X 50 mm, 1/pkg
  WAT038508	DeltaPak C4 Radial-Pak Cartridge, 100?, 15 µm, 25 mm X 100 mm, 1/pkg
  WAT034225	Shodex DE-G Guard Column, 4.6 x 10 mm
  WAT011808	DeltaPak C4 Prep Column, 100?, 15 µm, 7.8 mm X 300 mm, 1/pkg
  WAT011809	DeltaPak C4 Prep Column, 100?, 15 µm, 19 mm X 300 mm, 1/pkg
  WAT011633	DeltaPak C4 Radial-Pak Cartridge, 100?, 15 µm, 47 mm X 300 mm, 1/pkg